Boc-L-Isoleucine-[15N]

Boc-L-Isoleucine-[15N], an isotopically labeled amino acid derivative commonly utilized in research and development environments, offers a multitude of applications. Here are key applications presented with high perplexity and burstiness:

Protein Structure Analysis: A cornerstone in nuclear magnetic resonance (NMR) spectroscopy, Boc-L-Isoleucine-[15N] plays a pivotal role in unraveling protein structures. The incorporation of 15N enables researchers to meticulously track nitrogen atoms within polypeptides, facilitating the exploration of protein folding dynamics. This data is instrumental in deciphering protein functions and in the creation of novel pharmaceuticals.

Metabolic Flux Analysis: Within metabolic investigations, Boc-L-Isoleucine-[15N] acts as a powerful tracer for studying amino acid metabolism. Researchers introduce this labeled compound into cellular systems to scrutinize metabolic pathways and nitrogen atom flow. By examining the incorporation patterns of 15N, valuable insights into metabolic regulation and the enhancement of bioprocesses are uncovered.

Peptide Synthesis: In the realm of solid-phase peptide synthesis, Boc-L-Isoleucine-[15N] is harnessed for crafting isotopically labeled peptides. The incorporation of 15N facilitates the tracking of peptide bonds within synthetic peptides during analysis using mass spectrometry or NMR techniques. This methodology proves invaluable for investigating peptide interactions and stability in intricate biological systems.

Pharmacokinetic Studies: Enabling comprehensive exploration of drug absorption, distribution, metabolism, and excretion (ADME), Boc-L-Isoleucine-[15N] is a crucial component in pharmacokinetic analyses. By incorporating this compound into drug molecules, researchers can monitor nitrogen-containing fragments, enhancing their understanding of drug behavior within biological systems. This data plays a pivotal role in refining drug formulations and dosing strategies, ultimately optimizing therapeutic outcomes.