L-Leucine-[13C6,15N]

L-Leucine-[13C6,15N], an isotopically labeled amino acid, plays a pivotal role in a wide array of research applications aimed at tracing and comprehending metabolic processes. Here are the key applications of L-Leucine-[13C6,15N]:

Metabolic Flux Analysis: In the realm of metabolic research, L-Leucine-[13C6,15N] serves as a powerful tool for tracing leucine incorporation and unraveling metabolic pathways. By scrutinizing the distribution of the labeled isotopes, researchers can glean insights into protein synthesis rates, intermediary metabolism, and leucine catabolism. This crucial analysis is fundamental for comprehending metabolic disorders and optimizing strategies in metabolic engineering.

Protein Turnover Studies: Embarking on protein turnover studies, scientists utilize L-Leucine-[13C6,15N] to measure the rates at which proteins are synthesized and degraded within cells and tissues. By integrating the labeled leucine into newly formed proteins, researchers can meticulously track the dynamics of protein synthesis and degradation over time. This valuable information lends itself to the study of conditions such as muscle wasting diseases, processes of aging, and the impact of different treatments on protein metabolism.

Nutritional Research: In the domain of nutritional research, L-Leucine-[13C6,15N] emerges as a crucial asset for investigating amino acid metabolism and delineating dietary protein requirements. By leveraging this labeled amino acid, nutritionists can evaluate the efficiency of leucine utilization and the balance of amino acids in diverse dietary contexts. This analysis aids in formulating dietary supplements and therapeutic diets tailored to individuals with specific medical conditions or unique nutritional requirements.

Stable Isotope-Resolved Metabolomics (SIRM): Employed in stable isotope-resolved metabolomics, L-Leucine-[13C6,15N] enables researchers to delve into the dynamic alterations within metabolic networks. This innovative approach facilitates the quantification of metabolic fluxes and sheds light on the biochemical pathways of leucine in vivo. By harnessing this technique, scientists can investigate metabolic adaptations in various physiological and pathological states, thereby enhancing our understanding of disease mechanisms and unearthing potential therapeutic targets.