Boc-Leu-OH-[d10] monohydrate

Boc-Leu-OH-[d10] monohydrate, an isotopically labeled compound widely utilized in diverse bioscientific disciplines, plays a pivotal role in several key applications. Here are the applications:

Protein Synthesis Studies: Serving as a labeled amino acid in the realm of protein synthesis research, Boc-Leu-OH-[d10] monohydrate integrates into peptides and proteins, enabling intricate investigations using mass spectrometry-based methodologies. By monitoring and quantifying the assimilation of this amino acid into synthesized proteins, researchers delve into the nuances of protein dynamics and synthesis rates, enriching our understanding of molecular processes at play.

Metabolomics: The isotopic labeling facilitated by Boc-Leu-OH-[d10] monohydrate proves indispensable in the realm of metabolomics, offering a means to trace metabolic pathways with precision. Tracking the labeled leucine across various metabolic reactions unveils pathways and interactions with other metabolites, shedding light on cellular metabolism intricacies and unveiling insights into metabolic irregularities and aberrations.

Stable Isotope-Labeling Experiments: Positioned at the forefront of biochemical and biophysical exploration, Boc-Leu-OH-[d10] monohydrate finds its niche in stable isotope-labeling experiments. The presence of deuterium atoms within the compound amplifies the detectability and quantification of peptides and proteins, bolstering structural analyses and elucidating protein-protein as well as protein-ligand interactions. This avenue aids in unraveling molecular intricacies fundamental to various biological processes.

NMR Spectroscopy: Employed in NMR spectroscopy to unravel the structural dynamics of proteins, Boc-Leu-OH-[d10] monohydrate stands as a paramount tool in the exploration of protein folding and interactions. The discernible signal arising from the deuterium label facilitates the assignment of NMR resonances, fostering a profound comprehension of protein structure-function relationships. This endeavor significantly contributes to advancements in structural biology and innovations in drug discovery pursuits.