L-Methionine-[13C5,15N]

L-Methionine-[13C5,15N] a stable isotope-labeled amino acid finds applications across various bioscience domains. Here are the key applications of L-Methionine-[13C5,15N]:

Metabolic Flux Analysis: In the intricate realm of metabolic flux analysis, L-Methionine-[13C5,15N] plays a vital role in tracing and quantifying metabolic pathways. By integrating this labeled amino acid into metabolic investigations, researchers can meticulously follow the carbon and nitrogen atoms as they traverse through diverse metabolic reactions. This insightful approach aids in unraveling the dynamics of metabolism and pinpointing potential metabolic bottlenecks that may shape cellular function.

Protein Synthesis Studies: At the heart of protein synthesis studies lies the indispensable contribution of labeled methionine. By incorporating L-Methionine-[13C5,15N] into cell culture media, scientists can delve into the intricacies of protein synthesis and turnover dynamics. Leveraging sophisticated mass spectrometry techniques, researchers can precisely quantify the incorporation of this labeled amino acid into newly synthesized proteins, shedding light on the intricacies of protein regulation and cellular homeostasis.

Stable Isotope-Resolved Metabolomics: The cutting-edge realm of stable isotope-resolved metabolomics harnesses L-Methionine-[13C5,15N] to delve into metabolic alterations within cells and tissues. Through the strategic administration of this isotope-labeled compound to organisms, researchers can discern shifts in metabolic pathways under varied conditions. This approach proves invaluable for investigating disease states, drug impacts, and nutrient utilization patterns, offering a nuanced understanding of cellular metabolism and its response to external stimuli.

Nutrient Tracing in Ecological Studies: In the interdisciplinary field of ecological studies, L-Methionine-[13C5,15N] serves as a powerful tool for tracking nutrient flows across ecosystems. By introducing the labeled amino acid into soil or aquatic systems, scientists can monitor the intricate movement of nutrients through different trophic levels. This approach not only enhances our comprehension of nutrient cycling dynamics but also unveils the repercussions of environmental changes on ecosystem stability and resilience.