L-Methionine-[15N]

L-Methionine-[15N], an isotopically labeled amino acid, finds diverse applications in the realm of biosciences. Here are the key applications of L-Methionine-[15N]:

Protein Structure and Dynamics: Delving into the intricacies of protein science, L-Methionine-[15N] is a staple in nuclear magnetic resonance (NMR) spectroscopy. By incorporating this labeled amino acid into proteins, researchers embark on a journey to unveil the mysteries surrounding protein folding and interactions. The ability to track specific nitrogen atoms provides a window into the dynamic world of protein structure, offering crucial insights indispensable for deciphering protein functionality and unlocking potential therapeutic targets in the fight against protein misfolding disorders.

Metabolic Flux Analysis: Embarking on metabolic adventures, L-Methionine-[15N] serves as a beacon in tracing the pathways and fluxes of nitrogen within cellular metabolism. By introducing this isotopic tracer into cells or organisms, scientists set the stage to monitor the intricate dance of nitrogen through diverse metabolic routes. This journey of exploration sheds light on nitrogen utilization and turnover, crucial pillars for optimizing nutritional strategies and igniting innovations in metabolic engineering applications.

Quantitative Proteomics: In the realm of quantitative proteomics, L-Methionine-[15N] shines as a key player in stable isotope labeling by amino acids in cell culture (SILAC). This cutting-edge technique involves nurturing cells in media infused with L-Methionine-[15N], ushering in a realm where newly synthesized proteins bear the mark of this isotopic label. Researchers, armed with mass spectrometry, embark on a quest to compare the abundance of labeled and unlabeled proteins, paving the way for the identification and quantification of proteins entwined in biological processes and disease states, unraveling the intricate tapestry of proteomic landscapes.

Plant Physiology: Journeying into the green realms of plant physiology, L-Methionine-[15N] emerges as a beacon guiding investigations into nitrogen metabolism and amino acid biosynthesis in plants. By nourishing plants with this labeled amino acid, researchers embark on a voyage to trace the incorporation of nitrogen into an array of metabolic compounds. This journey fuels the understanding of how plants assimilate and distribute nitrogen, offering crucial insights for enhancing crop nutrition and fostering growth under the diverse tapestry of environmental conditions, nurturing a deeper understanding of plant life and its intricate relationship with nitrogen dynamics.