L-Methionine-[2-13C,15N]

L-Methionine-[2-13C,15N], a stable isotope-labeled amino acid, finds extensive use in research and development across diverse scientific fields. Here are key applications of L-Methionine-[2-13C,15N] presented with high perplexity and burstiness:

Metabolic Flux Analysis: A vital tool in metabolic flux analysis, L-Methionine-[2-13C,15N] aids in unraveling the intricate dynamics of metabolism within living organisms. Researchers leverage this labeled amino acid to meticulously trace and quantify the intricate flow of carbon and nitrogen through metabolic pathways. This knowledge forms the foundation for comprehending cellular metabolism, refining metabolic engineering strategies, and crafting innovative therapeutic approaches for metabolic disorders.

Protein Turnover Studies: Within the realm of proteomics, L-Methionine-[2-13C,15N] serves as a cornerstone for measuring protein turnover rates and examining protein dynamics within cellular and tissue environments. By integrating this labeled amino acid into newly synthesized proteins, scientists can track the intricate processes of protein synthesis and degradation over extended periods. This application is critical for investigating diseases linked to protein homeostasis, such as cancer and neurodegenerative disorders, shedding light on fundamental biological mechanisms.

Structural Biology: In the domain of structural biology, L-Methionine-[2-13C,15N] plays a pivotal role in nuclear magnetic resonance (NMR) spectroscopy and other advanced techniques aimed at unraveling protein structures. The strategic isotope labeling significantly enhances resolution and signal clarity in NMR studies, empowering researchers to decipher intricate protein folding patterns, interactions, and conformations. This capability is instrumental in advancing drug discovery efforts and fostering the development of cutting-edge protein-based therapeutics.

Nutritional Studies: In the realm of nutrition science, L-Methionine-[2-13C,15N] serves as a key asset for exploring amino acid metabolism and utilization in both human subjects and animal models. By meticulously tracing the metabolic destiny of methionine, researchers can glean insights into dietary requirements, grasp the complexities of metabolic disorders, and evaluate the impacts of nutritional interventions. This application forms the backbone for crafting optimized dietary regimes and managing conditions associated with methionine metabolism, such as homocystinuria, contributing to advancements in personalized nutrition strategies.