L-Methionine-[methyl-13C,d]

L-Methionine-[methyl-13C,d], a stable isotope-labeled derivative of methionine, is a valuable tool in scientific research. Here are key applications of L-Methionine-[methyl-13C,d] presented with high perplexity and burstiness:

Metabolic Flux Analysis: In the realm of systems biology, L-Methionine-[methyl-13C,d] plays a pivotal role in metabolic flux analysis. By introducing this isotope-labeled methionine into cellular cultures, researchers can unravel the intricate carbon pathways and quantify metabolic processes. This technique allows for in-depth explorations of metabolic network dynamics and the identification of critical metabolic bottlenecks.

Protein Turnover Studies: Delving into proteomics, L-Methionine-[methyl-13C,d] is a key player in investigating protein turnover dynamics. By labeling proteins with this stable isotope, scientists can probe into protein synthesis and degradation rates, shedding light on protein turnover and stability. This information is paramount in deciphering various disease states, including cancer and neurodegenerative disorders.

Methylation Research: The labeled methyl group in L-Methionine-[methyl-13C,d] serves as a powerful tool for delving into methylation processes, particularly in the realm of epigenetics and gene expression regulation. Researchers leverage this labeled methionine to trace methyl donors along biochemical pathways and to explore modifications on DNA and histones. This aids in comprehending the profound impact of methylation on gene expression patterns and cellular differentiation.

Biochemical Pathway Elucidation: In the exploration of biochemical pathways involving methionine, such as the transsulfuration and methylation cycles, L-Methionine-[methyl-13C,d] plays a crucial role in providing clarity. By offering insights into these intricate pathways, this stable isotope aids researchers in unraveling diseases associated with methionine metabolism, such as homocystinuria. Furthermore, it contributes to the exploration of regulatory mechanisms governing methionine-related enzymes, unlocking new realms of biochemical understanding.