L-Ornithine-[15N2] hydrochloride

L-Ornithine-[15N2] hydrochloride, a stable isotopically labeled compound, finds diverse applications in biochemical and clinical research. Here are the key applications:

Metabolic Flux Analysis: Delving into metabolic intricacies, L-Ornithine-[15N2] hydrochloride serves a pivotal role in metabolic flux analysis, tracing nitrogen assimilation and metabolism within cells. By introducing this labeled compound into culture media, researchers can meticulously track nitrogen fluxes through the urea cycle and other metabolic pathways, unveiling the dynamic nature of nitrogen metabolism and pinpointing metabolic bottlenecks.

Protein Turnover Studies: Unveiling the dynamic world of protein dynamics, L-Ornithine-[15N2] hydrochloride is a linchpin for studying protein turnover and synthesis rates in cells and tissues. Leveraging its isotopic label, researchers can precisely quantify protein degradation and synthesis by integrating it into newly formed proteins. This methodology is indispensable for comprehending the intricate dance of protein dynamics in both health and disease states.

Clinical Research: In the realm of clinical investigations, L-Ornithine-[15N2] hydrochloride emerges as a crucial tool for probing liver function and metabolic disorders. By using this compound, clinicians can evaluate the effectiveness of therapies targeting the urea cycle and ammonia detoxification, shedding light on metabolic alterations underlying conditions like hepatic encephalopathy and urea cycle disorders. This compound offers a window into the intricate metabolic landscape of clinical entities.

Amino Acid Metabolism Studies: Researchers harness the power of L-Ornithine-[15N2] hydrochloride to unravel the complex pathways of amino acid metabolism and their intricate regulation. By meticulously tracing the integration and conversion of this labeled ornithine, scientists can glean insights into the synthesis of arginine, proline, and polyamines. This application stands as a cornerstone for deciphering the nuances of amino acid biosynthesis and their multifaceted roles in various physiological processes, offering a deeper understanding of metabolic intricacies.