L-Phenylalanine-[13C9,15N]

L-Phenylalanine-[13C9,15N], a stable isotope-labeled amino acid, finds diverse applications in scientific and industrial fields. Here are the key applications.

Metabolic Flux Analysis: In the realm of metabolic flux analysis, L-Phenylalanine-[13C9,15N] emerges as a pivotal tool for delving into the intricate metabolic pathways within living cells. By integrating this labeled amino acid into cellular systems, researchers embark on a journey to trace and quantify the intricate flow of carbon and nitrogen atoms through diverse metabolic pathways. This endeavor unveils profound insights into enzyme activities, pathway dynamics, and the ebbs and flows of cellular metabolism.

Protein Structural Studies: Within the domain of structural biology, L-Phenylalanine-[13C9,15N] assumes a critical role in NMR (Nuclear Magnetic Resonance) spectroscopy. By incorporating this labeled amino acid into proteins, scientists gain access to a treasure trove of detailed information concerning protein structure and dynamics. This knowledge proves instrumental in unraveling the mysteries of protein folding, functionality, and intricate interactions with a myriad of other biomolecules.

Pharmaceutical Research: Positioned at the forefront of drug development and pharmacokinetics studies, L-Phenylalanine-[13C9,15N] emerges as a linchpin in the quest for novel therapeutics. By integrating the labeled amino acid into therapeutic proteins or peptides, researchers embark on a meticulous journey to monitor the distribution, metabolism, and ultimate fate of these compounds within the human body. Leveraging advanced techniques like mass spectrometry, this approach sheds light on optimizing drug formulations and decoding the intricate biological destiny of these therapeutic agents.

Nutritional Studies: Stepping into the realm of nutritional research, labeled amino acids like L-Phenylalanine-[13C9,15N] serve as indispensable tools for exploring amino acid metabolism and the utilization of dietary proteins. By meticulously tracking the integration and turnover of these labeled molecules within human or animal models, scientists gain valuable insights into protein quality, nitrogen balance, and the nuanced impacts of diverse dietary compositions on amino acid requirements. This information forms the cornerstone for crafting nutritional products and guidelines that cater to the diverse needs of individuals seeking to optimize their dietary intake.