L-Phenylalanine-[2,3-13C2,15N]

L-Phenylalanine-[2,3-13C2,15N], a stable isotope-labeled amino acid with diverse scientific applications, stands as a cornerstone across various fields. Here are the key applications of L-Phenylalanine-[2,3-13C2,15N]:

Metabolic Pathway Tracing: In the intricate realm of metabolic studies, L-Phenylalanine-[2,3-13C2,15N] assumes a pivotal role in tracing metabolic pathways and fluxes. By seamlessly integrating this labeled amino acid into biological systems, researchers unveil its intricate journey into an array of metabolites through cutting-edge techniques such as mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. This meticulous approach provides a profound grasp of amino acid metabolism and its intricate regulation across diverse physiological and pathological conditions.

Protein Structure and Dynamics: Venturing into the domain of structural biology, L-Phenylalanine-[2,3-13C2,15N] emerges as a critical tool for delving into protein structure and dynamics. The integration of this labeled amino acid into proteins unveils a universe of possibilities in multi-dimensional NMR spectroscopy, offering detailed insights into protein folding, conformational dynamics, and interactions with other molecular entities. This nuanced understanding serves as a foundational pillar for unraveling the mysteries of protein function and for shaping the landscape of innovative therapeutic design.

Nutritional Research: At the juncture of nutritional exploration, L-Phenylalanine-[2,3-13C2,15N] assumes a vital role in dissecting amino acid absorption, utilization, and protein synthesis within human and animal systems. By meticulously tracking the journey of labeled phenylalanine, researchers scrutinize the digestion and assimilation of dietary proteins into bodily tissues, shedding light on the optimization of dietary protocols and elucidating the repercussions of diverse protein origins on the vast panorama of health and disease.

Enzyme Mechanism Studies: Within the intricate realm of enzymology, L-Phenylalanine-[2,3-13C2,15N] emerges as a linchpin for unraveling the enigmatic mechanisms surrounding phenylalanine-metabolizing enzymes. Embedding the labeled amino acid into enzyme assays grants researchers a front-row seat to witness reaction kinetics, intermediate states, and product formation. This application serves as a guiding beacon for decoding the intricate functions of enzymes and for crafting inhibitors tailored to combat diseases associated with aberrant phenylalanine metabolism, such as phenylketonuria (PKU).