L-Proline-[d7,15N]

L-Proline-[d7,15N] is an isotopically labeled amino acid commonly used in various research applications. Here are some key applications of L-Proline-[d7,15N]:

Metabolic Pathway Tracing: L-Proline-[d7,15N] is utilized in metabolic studies to trace the incorporation and utilization of proline in metabolic pathways. By tracking the labeled isotope, researchers can understand the flux through proline-related metabolic networks. This helps in unraveling the metabolic dynamics in different physiological and pathological states.

Protein Structural Analysis: The isotopic labeling of L-Proline-[d7,15N] is invaluable in nuclear magnetic resonance (NMR) spectroscopy studies to elucidate protein structures and dynamics. The incorporation of deuterium and nitrogen-15 isotopes enhances signal clarity and resolution. This allows scientists to investigate the folding, interaction, and conformational changes of proteins with high precision.

Stable Isotope Labeling in Proteomics (SILAC): L-Proline-[d7,15N] is employed in SILAC experiments for quantitative proteomics. By incorporating this labeled amino acid into cellular proteins, researchers can perform precise quantitative comparisons of protein expression levels under different conditions. This technique is crucial for understanding cellular responses, identifying biomarkers, and studying disease mechanisms.

Nutritional Studies: L-Proline-[d7,15N] can be used in nutritional research to study the role of proline in diet and health. By administering the labeled amino acid in dietary studies, scientists can monitor its absorption, utilization, and metabolic fate. This helps in exploring the nutritional needs, metabolism, and potential therapeutic uses of proline in various health contexts.