L-Serine-[d3,13C3,15N]

L-Serine-[d3,13C3,15N] is a stable isotope-labeled compound used in various research and analytical applications. Here are some key applications of L-Serine-[d3,13C3,15N]:

Metabolic Flux Analysis: L-Serine-[d3,13C3,15N] is a valuable tool in metabolic flux analysis to study the pathways and rates of metabolism. By tracing the incorporation of labeled serine into metabolic pathways, researchers can quantify metabolic fluxes and understand the dynamics of serine metabolism. This provides detailed insights into cellular metabolism and aids in identifying metabolic disruptions in diseases.

Protein Structure and Function Studies: In protein research, L-Serine-[d3,13C3,15N] can be incorporated into proteins to investigate their structure and function using nuclear magnetic resonance (NMR) spectroscopy. By labeling specific residues, researchers can obtain detailed information on protein folding, dynamics, and interactions. This helps in understanding protein behavior and designing therapeutic interventions.

Stable Isotope Labeling: L-Serine-[d3,13C3,15N] is used in stable isotope labeling experiments to study cellular processes such as protein turnover, biosynthesis, and degradation. By evaluating the incorporation of the labeled amino acid into cellular proteins, scientists can monitor the synthesis and degradation rates of proteins. This technique is critical for understanding cellular homeostasis and disease mechanisms.

Biomarker Discovery: The labeled serine can be used in metabolomics and proteomics studies to identify and quantify biomarkers. By tracking changes in labeled serine metabolism in different physiological or pathological states, researchers can discover potential diagnostic markers for diseases. This aids in early detection, diagnosis, and understanding disease progression.