L-Threonine-[d5,15N]

L-Threonine-[d5,15N] is a stable isotope-labeled amino acid used primarily in bioscience research. Here are some key applications of L-Threonine-[d5,15N]:

Metabolic Flux Analysis: L-Threonine-[d5,15N] is used in metabolic flux analysis to trace metabolic pathways. By incorporating this labeled amino acid into a biological system, researchers can monitor its incorporation and conversion through various metabolic routes. This helps in understanding how metabolic networks function and identifying potential bottlenecks or regulatory points.

Protein Synthesis Studies: In protein synthesis research, L-Threonine-[d5,15N] assists scientists in investigating protein turnover and synthesis rates. The isotope labeling allows specific tracking of threonine incorporation into newly synthesized proteins. This technique is particularly useful in studying the dynamics of protein turnover under different physiological conditions or in response to treatments.

Nutritional Research: L-Threonine-[d5,15N] is employed in nutritional studies to evaluate amino acid metabolism and dietary requirements. By tracking the metabolism of the labeled threonine in humans or animals, researchers can gain insights into threonine utilization, absorption rates, and overall nitrogen balance. This information is crucial for determining nutritional needs and optimizing dietary formulations.

Chemical Proteomics: In chemical proteomics, L-Threonine-[d5,15N] is used to label proteins in living cells for mass spectrometry analysis. This approach allows for the detailed study of protein interaction networks and post-translational modifications. The precise labeling enhances the accuracy of quantitative proteomics, aiding in the identification of biomarkers and therapeutic targets.