L-Threonine-[d5]

L-Threonine-[d5] is an isotopically labeled amino acid used in various scientific and industrial applications. Here are some key applications of L-Threonine-[d5]:

Metabolic Flux Analysis: L-Threonine-[d5] is used in metabolic flux analysis to trace metabolic pathways and quantify fluxes in biological systems. By incorporating this labeled amino acid into metabolic studies, researchers can monitor its incorporation and turnover in various metabolic processes. This helps in understanding the dynamics and regulation of metabolic networks in organisms.

Protein Structure Analysis: In structural biology, L-Threonine-[d5] can be used in NMR spectroscopy and mass spectrometry for protein structure determination. The deuterium labeling provides enhanced resolution and better understanding of protein folding, interactions, and dynamics. This isotopic labeling is crucial for elucidating the conformational changes and binding sites of proteins.

Nutritional Studies: L-Threonine-[d5] is used in nutritional research to study amino acid metabolism and nutrient utilization. By administering this labeled amino acid, scientists can track its absorption, distribution, and incorporation into proteins. This application is essential for optimizing dietary formulations and understanding the role of specific amino acids in nutrition.

Pharmacokinetics: In pharmaceutical research, L-Threonine-[d5] is employed to investigate the pharmacokinetics of drugs and their metabolites. The isotopic labeling helps in distinguishing the administered compound from endogenous substances, allowing precise monitoring of drug absorption, distribution, metabolism, and excretion. This data is vital for drug development and optimizing therapeutic regimens.