L-Tyrosine-[1-13C,15N]

L-Tyrosine-[1-13C,15N], an isotopically labeled amino acid widely employed in diverse research settings, boasts a spectrum of key applications each:

Metabolic Flux Analysis: In the realm of metabolic flux analysis, L-Tyrosine-[1-13C,15N] serves as a pivotal tool for elucidating metabolic pathways and quantifying fluxes across various biochemical reactions. By integrating this labeled amino acid into cellular metabolism, researchers can meticulously track its conversion into diverse metabolites. This fosters a deep comprehension of the dynamics and regulation governing intricate metabolic networks.

Protein Structure and Dynamics: Within the domain of structural biology, L-Tyrosine-[1-13C,15N] finds utility in NMR spectroscopy, enabling an in-depth exploration of protein structure and dynamics. The isotopic labels play a crucial role in enhancing the detection and resolution of NMR signals, facilitating a detailed scrutiny of protein folding, interactions, and conformational changes. Such analyses shed light on protein function and mechanisms, enriching our understanding of cellular processes.

Proteomics: Enlisted in quantitative proteomics, L-Tyrosine-[1-13C,15N] emerges as a key player in accurately measuring protein abundances. Leveraging mass spectrometry, researchers can discern between labeled and unlabeled tyrosine residues within proteins, enabling precise quantification. This precision proves critical for investigating diverse aspects such as post-translational modifications, protein turnover rates, and the differential expression of proteins, paving the way for comprehensive proteomic analyses.

Pharmacokinetics: Stepping into the realm of pharmacokinetic studies, the labeled L-Tyrosine-[1-13C,15N] takes center stage in unraveling the absorption, distribution, metabolism, and excretion (ADME) dynamics of drugs and nutrients. By monitoring the trajectory of the labeled amino acid within biological systems, researchers gather invaluable insights into how substances interact within the body. This knowledge facilitates advancements in drug development and personalized medicine, underscoring the pivotal role of isotopic labeling in pharmacokinetic research.