L-Tyrosine-[13C9,15N] is an isotopically labeled amino acid used extensively in research and analytical applications. Here are some key applications of L-Tyrosine-[13C9,15N]:
Metabolic Flux Analysis: L-Tyrosine-[13C9,15N] is employed to trace and quantify metabolic pathways in cells. By incorporating this labeled tyrosine into metabolic studies, researchers can track the flow of carbon and nitrogen through various pathways. This detailed insight is crucial for understanding cellular metabolism and identifying metabolic bottlenecks.
Protein Structure Elucidation: This isotopically labeled amino acid is an invaluable tool in NMR spectroscopy for protein structure determination. The incorporation of L-Tyrosine-[13C9,15N] into proteins allows researchers to observe specific labeling patterns and distinguish between different amino acid residues. This enhances the resolution and accuracy of protein structure models.
Quantitative Proteomics: L-Tyrosine-[13C9,15N] is used in mass spectrometry-based proteomics to quantify protein expression levels with high precision. By integrating this labeled amino acid into peptide labeling approaches, researchers can compare the abundance of proteins across different samples. This technique is crucial for biomarker discovery and understanding disease mechanisms.
Pathway Engineering: In synthetic biology and metabolic engineering, L-Tyrosine-[13C9,15N] can be used to monitor the efficiency of engineered metabolic pathways. By feeding this labeled amino acid to genetically modified organisms, scientists can evaluate the production and conversion rates of target metabolites. This aids in optimizing pathway constructs for industrial applications such as biofuel and pharmaceutical production.
![L-Tyrosine-[13C9,15N]](https://resource.bocsci.com/structure/202407-26-9.gif)
