Name: L-Valine-[13C5,15N]
Brand: BOC Sciences
Rating: 5 (1 reviews)

General Information

Catalog: BLP-009362

CAS: 202407-30-5

Molecular Formula: [13C]5H11[15N]O2

Molecular Weight: 123.10

Chemical Structure

Description	L-Valine-[13C5,15N] is a labelled L-Valine. Valine is a branched-chain amino acid used in the protein biosynthesis. It is essential for animals with insulin-resistant property.
Synonyms	(+)-2-Amino-3-methylbutyric Acid-13C5,15N; (2S)-2-Amino-3-methylbutanoic Acid-13C5,15N; (S)-2-Amino-3-methylbutanoic Acid-13C5,15N; (S)-Valine-13C5,15N; (S)-α-Amino-β-methylbutyric Acid-13C5,15N; L-(+)-α-Aminoisovaleric Acid-13C5,15N; L-α-Amino-β-methylbutyric Acid-13C5,15N; NSC 76038-13C5,15N
IUPAC Name	(2S)-2-(15N)azanyl-3-(1-13C)methyl(1,2,3,4-13C4)butanoic acid
Related CAS	72-18-4 (unlabelled)
Isomeric SMILES	[13CH3][13CH]([13CH3])[13C@@H]([13C](=O)O)[15NH2]
Canonical SMILES	CC(C)C(C(=O)O)N
InChI	InChI=1S/C5H11NO2/c1-3(2)4(6)5(7)8/h3-4H,6H2,1-2H3,(H,7,8)/t4-/m0/s1/i1+1,2+1,3+1,4+1,5+1,6+1
InChI Key	KZSNJWFQEVHDMF-XAFSXMPTSA-N
Melting Point	295-300°C (subl.) (lit.)
Purity	98% by HPLC; 99% atom 13C, 99% atom 15N
Solubility	Soluble in Aqueous Acid (Slightly), Water (Slightly, Heated)
Appearance	White to Off-white Solid
Storage	Store at RT

L-Valine-[13C5,15N] is a stable isotope-labeled amino acid used in a variety of bioscience applications. Here are some key applications of L-Valine-[13C5,15N]:

Metabolic Flux Analysis: L-Valine-[13C5,15N] is employed in metabolic flux analysis to study cellular metabolism and pathways. By tracking the incorporation of stable isotopes into metabolic products, researchers can quantify metabolic rates and fluxes in cells. This technique provides insights into metabolic regulation and bioengineering strategies.

Protein Structure and Dynamics: In NMR (Nuclear Magnetic Resonance) spectroscopy, L-Valine-[13C5,15N] serves as a labeled amino acid to study the structure and dynamics of proteins. The incorporation of the isotopes enhances the sensitivity and resolution of NMR signals, facilitating detailed analysis of protein folding interactions and conformational changes. This is crucial for understanding protein function and designing novel therapeutics.

Quantitative Proteomics: L-Valine-[13C5,15N] can be used in quantitative proteomics to measure protein abundance and turnover rates. By incorporating the labeled amino acid into cell cultures, researchers can perform mass spectrometry-based quantification of proteins with high precision. This approach aids in the discovery of biomarkers and the study of protein regulation under different conditions.

Nutritional Studies: In nutritional research, L-Valine-[13C5,15N] is used to study amino acid metabolism and dietary requirements. By administering the labeled amino acid to subjects, scientists can track its utilization and distribution in the body. This helps in understanding metabolic disorders, optimizing dietary formulations, and developing nutritional therapies.