L-Valine-[d8,13C5,15N]

L-Valine-[d8,13C5,15N], a labeled amino acid utilized in diverse scientific research applications, harnesses its isotopic enrichment for intricate analytical and experimental purposes. Here are the key applications of L-Valine-[d8,13C5,15N]:

Metabolic Flux Analysis: Delving into metabolic pathways, L-Valine-[d8,13C5,15N] acts as a trailblazer tracing isotopic labels across various metabolites. This methodology unveils the intricacies of metabolic networks, pinpointing bottlenecks and regulatory nodes within cellular metabolism. Researchers quantify the diverse contributions of pathways to overall metabolic flux, propelling our comprehension of cellular physiology to new heights.

Proteomics: Within the realm of proteomics, L-Valine-[d8,13C5,15N] assumes the role of a heavy isotope label, facilitating precise quantitative mass spectrometry analysis. By integrating the labeled valine into proteins, scientists meticulously compare protein expression levels under varying conditions. This precision unravels biomarkers and elucidates the molecular underpinnings of diverse diseases, shedding light on intricate cellular mechanisms.

Structural Biology: Navigating the landscape of structural biology, L-Valine-[d8,13C5,15N] emerges as a vital ally in NMR spectroscopy and other structural analysis methodologies. The isotopic labels amplify the resolution and sensitivity of NMR spectra, simplifying the exploration of protein folding dynamics, interactions, and conformational shifts. This exploration deepens our grasp of protein function and stability, unraveling the mysteries of molecular architecture.

Nutritional Studies: In the realm of nutritional research, L-Valine-[d8,13C5,15N] stands out as a crucial asset for tracking amino acid utilization and metabolism in organisms. Administering the labeled valine, researchers observe its assimilation into proteins, evaluating the efficiency of dietary protein absorption and utilization. This data is foundational for crafting optimized nutritional strategies and understanding the nuances of amino acid requirements across diverse physiological states.