Boc-Val-OH-[15N]

Boc-Val-OH-[15N], a nitrogen-15 labeled protected amino acid, finds wide application in biochemical and pharmaceutical research. Here are key applications presented with high perplexity and burstiness:

Isotope-Labeled Protein Studies: The utilization of Boc-Val-OH-[15N] in synthesizing isotope-labeled proteins for nuclear magnetic resonance (NMR) spectroscopy enables researchers to delve into the dynamics and interactions of protein structures with unparalleled depth. By introducing this compound, scientists can explore protein behavior in intricate biological systems at a granular level, thanks to the precision offered by nitrogen-15 labeling.

Drug Metabolism and Pharmacokinetics: In the realm of pharmaceutical research, Boc-Val-OH-[15N] plays a crucial role in elucidating the metabolism and pharmacokinetics of drugs containing proline or valine residues. The incorporation of stable isotope labeling offers a means to meticulously trace and quantify metabolites, enhancing comprehension of metabolic pathways. This advancement aids in refining drug formulations, predicting interactions, and foreseeing potential side effects.

Peptide Synthesis: The significance of Boc-Val-OH-[15N] shines in peptide synthesis, particularly for creating peptides with site-specific isotopic labeling for biochemical investigations. Its protected nature allows seamless integration into peptide chains while preserving stability until deprotection. This capability empowers researchers to explore peptide interactions, folding dynamics, and functional attributes with heightened resolution and specificity.

Biological Pathway Analysis: Engaging Boc-Val-OH-[15N] in metabolic pathway analysis and flux studies offers a window into nitrogen flow within valine residues of organisms, facilitating the tracking of metabolic networks. This methodology delivers invaluable insights into amino acid utilization, biosynthesis pathways, and the effects of genetic alterations or environmental influences on metabolic processes, enriching our understanding of biological pathways.