L-Valine-[d8,15N]

L-Valine-[d8,15N] is an isotopically labeled amino acid used in various advanced research applications. Here are some key applications of L-Valine-[d8,15N]:

Metabolic Flux Analysis: L-Valine-[d8,15N] is utilized in metabolic flux analysis to trace the pathways and rates of metabolic reactions. The isotopic labeling enables precise measurement of valine uptake and conversion in cellular metabolism. This information aids researchers in understanding metabolic disorders and optimizing metabolic engineering practices.

Proteomics: In proteomics studies, L-Valine-[d8,15N] serves as an internal standard for quantitative mass spectrometry. By incorporating this labeled amino acid into proteins, scientists can accurately quantify protein synthesis and degradation rates. This technique is essential for profiling protein dynamics in various biological contexts, including disease states and developmental processes.

NMR Spectroscopy: L-Valine-[d8,15N] is employed in nuclear magnetic resonance (NMR) spectroscopy to study protein structure and dynamics. The isotopic labeling enhances the resolution and sensitivity of NMR signals, facilitating detailed analysis of protein folding, interactions, and conformational changes. This allows for a deeper understanding of protein function and mechanism.

Nutritional Studies: L-Valine-[d8,15N] is used to investigate amino acid metabolism and nutritional requirements in organisms. By measuring the incorporation of this labeled amino acid into body tissues, researchers can assess the efficiency of protein synthesis and the impact of dietary valine on health. These studies are crucial for developing optimized nutritional strategies and dietary supplements.