Isotope Labeled Fatty Acids & Lipids
Product List
BOC Sciences is committed to providing customers with the most comprehensive variety of isotope-labeled fatty acids & lipids available. All our off-the-shelf or customized isotope-labeled fatty acids and lipids products are supplied with quality analysis reports, and certain products are provided with NMR, MS, HPLC, GC, as well as other spectra and detection techniques. The isotope-labeled fatty acids and lipids we offer include saturated fatty acids (such as stearic acid, palmitic acid, etc.), unsaturated fatty acids (such as oleic acid, linoleic acid, linolenic acid, etc.) and phospholipids. In addition, we also provide custom isotope labeling service for any fatty acids and lipids, you can directly provide the fatty acids or lipids to be labeled or make a request such as the type of labeling isotope, the labeling site, and the isotope enrichment.
What are Fatty Acids?
Fatty acids are long aliphatic hydrocarbon chains containing a carboxyl group at one end, and are organic matter, colorless, odorless, tasteless, and lipids with a relative density of less than one. In the presence of sufficient oxygen supply, fatty acids can be oxidized and decomposed into carbon dioxide and water, releasing a large amount of energy, so it is one of the body's main sources of energy. There are about 40 different fatty acids in nature, and they are the key components of lipids. The physical properties of many lipids depend on the degree of saturation of the fatty acid and the length of the carbon chain, where only fatty acids with an even number of carbon atoms can be absorbed and utilized by the body. Short-chain fatty acids can be categorized according to their structure, properties and nutritional value to the human body.
Non Essential Fatty Acids
Non-essential fatty acids are fatty acids that the body can synthesize on its own and does not have to rely on food supply, which includes saturated fatty acids and some monounsaturated fatty acids.
Essential Fatty Acids (EFA)
Essential fatty acids are necessary for human health and life, but the body can not synthesize their own, must rely on food supply, including linoleic acid, α-linolenic acid, are polyunsaturated fatty acids (PUFA).
What are Lipids?
Lipids, also known as fats, refer to substances that are soluble in organic solvents but insoluble in water. They encompass fats, phospholipids, and their derivatives. The chemical essence of lipids is esters formed by fatty acids and alcohols, along with their derivatives. Lipids are an important class of biomolecules with structural diversity. Lipid compounds can provide rich information, such as the source of organic matter, which is significant for studying life activities, chemical processes, and environmental changes. Lipids encompass a diverse group of biomolecules that include triglycerides, phospholipids, sterols, and sphingolipids, among others. These molecules are integral to cellular membranes, energy storage, and signaling pathways. Lipids exhibit remarkable structural diversity, contributing to the functional versatility observed across biological membranes and lipid-based signaling networks.
What are Isotope Labeled Fatty Acids & Lipids?
Isotope labeled fatty acids are synthetic or naturally occurring fatty acids where stable isotopes such as 13C or deuterium (2H) replace common isotopes in the carbon backbone. This substitution allows researchers to trace the fate of these fatty acids in biological systems using mass spectrometry. Isotope labeled fatty acids are invaluable tools for studying lipid metabolism dynamics, including synthesis rates, turnover rates, and lipid fluxes within cells and organisms.
Isotope labeled lipids extend the concept of isotope labeling beyond fatty acids to include entire lipid classes. By introducing stable isotopes into lipids, researchers can precisely track the biosynthesis, transport, and metabolism of specific lipid species. This approach provides insights into lipid remodeling processes and their regulation under various physiological and pathological conditions.
Our Isotope Labeling Services for Fatty Acids & Lipids
BOC Sciences offers a comprehensive Custom Isotope Labeled Fatty Acids Service tailored to meet the specific needs of researchers and pharmaceutical developers. Leveraging our expertise in organic synthesis and analytical chemistry, we provide high-quality isotope labeled fatty acids with customizable labeling patterns and purities. Our service supports diverse applications in lipidomics, including stable isotope labeling by amino acids in cell culture (SILAC) and stable isotope labeling by essential fatty acids in cell culture (SILFAC), among others.
Types of Isotopic Labeling We Offer
Isotopic Labeling Methods We Offer
| Isotopic Labeling Methods | Description | Inquiry |
|---|
| Single Isotope Labeling | Incorporation of a single stable isotope (e.g., 13C) into fatty acids or lipid classes for straightforward metabolic tracing. | Inquiry |
| Multiple Isotope Labeling | Introduction of multiple stable isotopes (e.g., 13C and 2H) to elucidate complex lipid metabolic pathways and turnover rates. | Inquiry |
| Uniform Labeling | Uniform labeling of lipid precursors to achieve consistent and high labeling efficiencies across entire lipidomes, essential for accurate quantitation and flux analysis. | Inquiry |
For more types of fatty acids & lipids (and their derivatives) and isotopic labeling, please contact us directly.
What are Stable Isotope Labeling by Fatty Acids in Cell Culture (SILFAC)?
Stable Isotope Labeling by Fatty Acids in Cell Culture (SILFAC) is a specialized technique used in lipidomics to study lipid metabolism dynamics within cultured cells. It involves supplementing cell culture media with stable isotope labeled fatty acids, such as 13C-labeled fatty acids or deuterated fatty acids (2H), which replace the natural, non-labeled fatty acids normally present in the media.
Key Aspects of SILFAC
Experimental Setup
Cells are cultured in a medium enriched with stable isotope labeled fatty acids. These labeled fatty acids serve as metabolic tracers that integrate into cellular lipid pools during lipid biosynthesis pathways.
Metabolic Tracing
By tracking the fate of these labeled fatty acids using analytical techniques like mass spectrometry, researchers can quantify the incorporation of labeled fatty acids into different lipid classes, such as phospholipids, triglycerides, and cholesterol esters.
Quantitative Analysis
SILFAC allows for precise measurement of lipid synthesis rates, turnover rates, and lipid remodeling processes within cells. It provides quantitative insights into how cells utilize exogenous fatty acids for membrane biogenesis, energy storage, and signaling.
What are Isotope Labeled Fatty Acids & Lipids Used For?
Isotope labeled fatty acids and lipids play pivotal roles across various scientific disciplines, offering unique insights into lipid metabolism, cellular dynamics, and disease mechanisms. Here are some key applications of isotope labeled fatty acids and lipids:
Metabolic Flux Analysis
Isotope labeled fatty acids are crucial for metabolic flux analysis in lipidomics. They serve as metabolic tracers to track the fate of fatty acids within biological systems. By introducing labeled fatty acids into cells or organisms and analyzing their incorporation into lipid pools using techniques like mass spectrometry, researchers can quantify metabolic fluxes, turnover rates, and pathways involved in lipid metabolism.
Cellular Lipid Dynamics
Studying lipid dynamics in cells and tissues is essential for understanding cellular functions and responses. Isotope labeled lipids allow researchers to investigate lipid synthesis, turnover, and degradation processes. This is particularly valuable in studying lipid rafts, membrane biogenesis, and lipid signaling pathways implicated in various physiological and pathological conditions.
Disease Mechanisms
Isotope labeled fatty acids and lipids are instrumental in elucidating lipid alterations associated with diseases such as metabolic disorders, cardiovascular diseases, neurodegenerative diseases, and cancer. By comparing the lipidomic profiles of healthy and diseased tissues, researchers can identify biomarkers, understand disease progression, and explore therapeutic targets related to lipid metabolism.
Drug Development and Pharmacology
In drug development, isotope labeled fatty acids are used to assess the effects of pharmaceuticals on lipid metabolism. They provide insights into how drugs influence lipid synthesis, transport, and storage within cells. This information aids in optimizing drug efficacy, evaluating drug safety profiles, and developing lipid-targeted therapies for metabolic diseases and lipid-related disorders.
Why Choose BOC Sciences to Provide You with Isotope Labeled Fatty Acids & Lipids?
- Expertise and Experience: With over two decades of experience in chemical synthesis and analytical services, BOC Sciences delivers precise and reliable isotope labeled fatty acids and lipids.
- Customization: We offer flexible customization options to tailor labeling patterns, purities, and quantities according to specific research requirements.
- Quality Assurance: Rigorous quality control measures ensure high purity and consistency of our isotope labeled products, meeting the rigorous standards of biological research.
- Collaborative Partnerships: We collaborate closely with researchers worldwide to advance lipidomics and support groundbreaking discoveries in lipid metabolism and related fields.
Isotope labeled fatty acids and lipids represent indispensable tools for unraveling the complexities of lipid metabolism in health and disease. By offering customizable isotope labeling services, BOC Sciences empowers researchers to delve deeper into lipidomic investigations, driving innovation and accelerating scientific breakthroughs. Whether probing lipid fluxes in cellular models or elucidating lipid signatures in preclinical samples, our commitment to excellence ensures that researchers have the precise tools needed to navigate the intricate pathways of lipid biology.
FAQ
1. What is the function of lipids?
Lipids are important components of cellular function and play a role in cell membrane formation, intercellular signaling, energy storage, and maintenance of homeostasis. Fat is not only a good energy storage material in the cell in addition to the role of insulation, cushioning and pressure reduction. Phospholipids are the main components that make up cell and organelle membranes. Sterols include cholesterol, sex hormones and vitamin D. Vitamin D effectively promotes the absorption of calcium and phosphorus from the intestines of humans and animals.
2. What is the monomer lipids?
Monomers of lipids are actually the basic units that make up lipids, and these can be fatty acids and esters formed from glycerol. These simple organic molecules are the basic building blocks of lipids and can be further combined with other molecules such as phosphoric acid and nitrogenous bases (e.g. choline, ethanolamine) to form more complex lipid molecules such as phospholipids.
3. What are unsaturated lipids?
Unsaturated lipids are lipids that contain one or more double bonds within the fatty acid chains. These double bonds introduce kinks in the fatty acid chains, preventing the molecules from packing tightly together. This structural feature gives unsaturated lipids distinct physical and chemical properties compared to saturated lipids, which have no double bonds and are typically more rigid.
4. What are omega 3 fatty acids?
Omega-3 fatty acids are essential polyunsaturated fatty acids that cannot be synthesized by the human body and must be consumed through food. It mainly includes alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).
5. What are essential fatty acids?
The essential fatty acids for the human body are linoleic acid (ω-6) and alpha-linolenic acid (ω-3). Derivatives of these essential fatty acids serve multiple physiological functions. DHA (ω-3) and ARA (ω-6) are the predominant fatty acids in brain, neural tissues, and the retina, playing crucial roles in the development of brain and visual functions.
6. What do omega 3 fatty acids do?
Omega-3 fatty acids play an important role in the human body. EPA (eicosapentaenoic acid) is lipophilic and has a stable extended conformation, which helps maintain membrane structure stability and uniform cholesterol distribution. It also regulates inflammation and endothelial dysfunction, inhibits free radicals and lipid oxidation, which is beneficial for reducing triglycerides, protecting cardiovascular and cerebrovascular health, and reducing inflammation damage. DHA (docosahexaenoic acid) is abundant in neural tissues and significantly impacts neuron and retinal membrane tissues, aiding in the improvement of cognitive functions, memory, and concentration. ALA (alpha-linolenic acid) must be converted into EPA and DHA in the body to be utilized effectively.
