Isotope Labeled Nucleic Acids
Product List
Nucleic acids, which are found in the cells of everybody that we examine and analyze is so fundamental for molecular biology, biochemistry as well as genetics. It is vital for improving scientific understanding and aiding new technology development to understand more about the structure, function and dynamics of these key biomolecules. In this context isotope-labeled nucleic acids are of paramount importance and have been used in detailed studies employing Nuclear Magnetic Resonance (NMR) spectrum as a technique. For over two decades, BOC Sciences has been a leading lab service provider in isotope labeling for up-to-date research. These are specifically concerns of the isotope-labeled nucleic acid-related products, and include internal isotopic standards for purine/pyrimidine/ inosine/ uridine/cytidine/thymidine/guanoshie derivatives. In addition, we also provide custom isotope labeling service for any nucleic acid and its derivatives, you can directly provide the nucleic acid to be labeled or make a request such as the type of labeling isotope, the labeling site, and the isotope enrichment.
What are Nucleic Acids?
Nucleosides consist of a pentose sugar (ribose or deoxyribose) and a nitrogenous base (pyrimidine or purine) linked by a glycosidic bond. Nucleosides can be categorized into ribonucleosides and deoxyribonucleosides by their pentose sugars. According to the different nitrogenous bases, nucleosides could be divided into adenosine, thymidine, uridine, guanosine, cytidine, inosine, etc. Nucleosides and phosphates synthesize nucleotides, and four unique nucleotides form the basis of nucleic acids. Nucleic acids are macromolecular compounds polymerized by monomeric nucleotides and are functional molecules with large molecular weights, including DNA and RNA. Their main functions are include storage, transmission, and performance of genetic information and are the protagonist of modern molecular biology. Nucleic acids are widely used to support the development of medicine, food, cosmetics, agriculture, etc.
What Is the Function of Nucleic Acids?
- In medicine: Nucleic acid drugs are a novel class of biopharmaceuticals, which have broad prospects in anti-cancer, anti-virus, treatment of cerebrovascular diseases, prevention of hypertension, and treatment of anemia.
- In food: Nucleic acid derivatives can be used as umami agents.
- In cosmetics: Nucleic acid has the effect of promoting protein synthesis and upregulating skin metabolism.
- In agriculture: Nucleic acid derivatives could be used as prevention for plant diseases and insect pests.
What Are Isotope-Labeled Nucleic Acids?
Isotope-labeled nucleic acids are molecules in which certain atoms have been replaced with their stable isotopes, such as carbon-13 (13C), nitrogen-15 (15N), or deuterium (2H). These isotopes have different nuclear properties, making them useful for various analytical techniques, especially NMR spectroscopy. Isotope labeling enhances the resolution and sensitivity of these techniques, allowing for more detailed structural and functional studies of nucleic acids. Frequently used nucleosides (such as uridine, thymidine, etc.) can be used for nucleic acid labeling, as well as chromosomal in situ hybridization or electron microscopy nucleic acid analysis. Stable isotopes are used in complex environments to trace the DNA/RNA of microorganisms and study metabolic functions, which significantly strengthen scientific research development and application value.
Our Isotope Labeling Services for Nucleic Acids
At BOC Sciences, we offer comprehensive isotope labeling services for nucleic acids tailored to meet the diverse needs of our clients in research, diagnostics, and therapeutic development. Our expertise and advanced technologies ensure high-quality, reliable, and customizable labeling solutions.
Custom Isotope Labeling Solutions
Our custom isotope labeling services are designed to provide flexibility and precision. We cater to specific project requirements, offering various labeling options including:
- Uniform Labeling: Incorporating isotopes uniformly throughout the nucleic acid molecule.
- Site-Specific Labeling: Targeting specific nucleotides or regions within the nucleic acid sequence for precise isotope incorporation.
- Segmental Labeling: Labeling distinct segments of a nucleic acid molecule, ideal for structural studies and interaction analyses.
Labeling of Different Nucleic Acid Types
We specialize in the isotope labeling of various nucleic acid types, including:
| Nucleic Acid Types | Description | Inquiry |
|---|
| DNA | Both single-stranded (ssDNA) and double-stranded (dsDNA) DNA molecules can be labeled to facilitate detailed structural and functional studies. | Inquiry |
| RNA | Our services include labeling of different RNA types, such as mRNA, tRNA, rRNA, and non-coding RNAs. We also provide labeling for RNA synthesized through in vitro transcription or chemical synthesis. | Inquiry |
| Oligonucleotides | Short DNA or RNA sequences used in various applications, including probes, primers, and therapeutic agents, can be precisely labeled. | Inquiry |
Choosing for Isotopic Labels
For more types of nucleic acids (and their derivatives) and isotopic labeling, please contact us directly.
Applications of Isotope Labeled Nucleic Acids
Isotope-labeled nucleic acids have a wide range of applications in scientific research and development:
- Structural Biology: NMR spectroscopy and X-ray crystallography benefit from isotope labeling, allowing for detailed structural analysis of nucleic acids and their complexes.
- Molecular Dynamics: Isotope labeling helps study the dynamic behavior of nucleic acids, including folding, conformational changes, and interactions with other biomolecules.
- Metabolic Pathway Analysis: Tracking the incorporation and metabolism of nucleic acids within cells using isotopic labels provides insights into cellular processes and biochemical pathways.
- Drug Development: Isotope-labeled nucleic acids are used in the development and testing of nucleic acid-based therapeutics, such as antisense oligonucleotides, aptamers, and small interfering RNAs (siRNAs).
- Diagnostic Probes: Labeled nucleic acids are employed as probes in diagnostic assays to detect specific nucleic acid sequences associated with diseases.
Why Choose BOC Sciences for the Synthesis of Your Isotope Labeled Nucleic Acids?
BOC Sciences stands out as a leader in isotope labeling services for nucleic acids due to our:
- Expertise and Experience: With over 20 years in the industry, our team of specialists brings extensive knowledge and technical proficiency to every project.
- Customization and Flexibility: We offer tailored solutions to meet the unique requirements of each client, ensuring the best possible outcomes.
- Advanced Technologies: Our state-of-the-art facilities and cutting-edge techniques guarantee high-quality and reliable labeling services.
- Commitment to Quality: We adhere to stringent quality control measures to ensure the accuracy and consistency of our labeled nucleic acids.
- Customer Support: Our dedicated support team is always available to assist clients throughout the entire process, from initial consultation to final delivery.
FAQ
1. What is the Monomer of Nucleic Acids?
The monomer of nucleic acids is the nucleotide. Each nucleotide consists of a nitrogenous base, a sugar molecule (deoxyribose in DNA and ribose in RNA), and a phosphate group.
2. What Do Nucleic Acids Do?
Nucleic acids store and transmit genetic information, guide the synthesis of proteins, regulate gene expression, and participate in various cellular processes.
3. Is DNA a Nucleic Acid?
Yes, DNA (deoxyribonucleic acid) is one of the two main types of nucleic acids. It carries the genetic blueprint of an organism.
4. What Are the Two Types of Nucleic Acids?
The two main types of nucleic acids are DNA (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid).
5. How Are Proteins and Nucleic Acids Related?
Proteins and nucleic acids are related through the central dogma of molecular biology, where DNA is transcribed into RNA, and RNA is translated into proteins. Proteins are synthesized based on the genetic code carried by nucleic acids.
6. Is ATP a Nucleic Acid?
ATP (adenosine triphosphate) is not a nucleic acid but a nucleotide. It serves as an energy carrier in cells and is composed of adenine, ribose (a sugar), and three phosphate groups.
