Relying on a professional technical team and high-quality and efficient services, BOC Sciences provides stable isotope labeling with amino acids in cell culture (SILAC) services for cell culture amino acids, and can meet the needs of different isotope-labeled amino acids required for experiments.
Service Introduction
Stable isotope labeling with amino acids in cell culture (SILAC) technology is based on the principle that cell growth depends on essential amino acids, and uses stable isotope-labeled (13C, 15N, 2H) essential amino acids to metabolically label cellular proteins.
13C, 15N, 2H are stable isotopes without radioactivity, so they can be tested under normal conditions. Arginine, lysine, and leucine are the most commonly used amino acids in SILAC. Stable isotope-labeled arginine, lysine, and leucine were added to the cell culture medium to culture the cells. These stable isotope-labeled amino acids are naturally incorporated into proteins during cell proliferation. After 5-6 generations of cell proliferation, the stable isotope-labeled amino acid is completely incorporated into the newly synthesized protein of the cell to replace the original amino acid.
SILAC technology labels proteins through the cell's normal metabolic processes. The labeled protein differs in molecular weight from unlabeled protein (incubated in normal medium), but other chemical properties are the same. This difference is distinguished by means of subsequent mass spectrometry. Relative quantitative research was carried out by comparing the peak area of the signal peaks of unlabeled and isotope-labeled samples in the first-order mass spectrometry. At the same time, protein identification can also be carried out by secondary mass spectrometry.
Technical Features
- In vivo labeling technology is closer to the real state of the sample.
- The effect is stable, and the labeling efficiency is as high as 99%.
- Incorporate markers during cell culture to reduce experimental errors.
- Accurate quantification, and wide linear range.
- Compared with chemical labeling, it has higher sensitivity and requires less protein.
- Hundreds to thousands of proteins can be identified and quantified simultaneously.
Application Scopes
- Drug target research
- Study on dynamic changes of protein expression in cultured cells or mice
- Screening for disease markers
- Quantitative comparative analysis of differential expression profiles
- Quantitative screening of molecular interactions (protein-protein interactions, RNA-protein interactions, DNA-protein interactions, small molecule-protein interactions, etc.)
- Quantitative Screening for Changes in Protein Post-Translational Modifications
- Large-scale modifier omics (methylation, phosphorylation, acetylation, etc.)
Service Content
- Culture medium preparation
- Isotope labeling of cells and detection of labeling efficiency by mass spectrometry
- Labeled and unlabeled cell expansion
- Protein extraction, separation (SDS-PAGE) and staining
- LC-MS analysis after digestion
- Mass spectrometry data analysis, protein identification and quantitative analysis, differentially expressed protein screening
- Routine bioinformatics analysis
- Experiment report submission
Service Advantages
- Rich service experience
- Appropriate SILAC protocol design
- Optimal experimental route
- Competitive price
- Complete Experimental Analysis Report
- Intimate after-sales service
Customer Notice
Before starting the experiment, we will wholeheartedly provide you with an effective SILAC experimental design plan. We can provide stable isotope-labeled amino acids.
The customer provides the sample and sample information to be identified, and the sample should avoid all kinds of pollution and repeated freezing and thawing.
Professional SILAC technology platform
- Diversified SILAC products
- Diversified SILAC services
