Stable isotope-labeled compounds are used as environmental pollutant standards for the detection of air, water, soil, sediment and food.
In addition to treating various diseases, isotopes are used for imaging, diagnosis, and newborn screening.
Small molecule compounds labeled with stable isotopes can be used as chemical reference for chemical identification, qualitative, quantitative, detection, etc. Various types of NMR solvents can be used to study the structure, reaction mechanism and reaction kinetics of compounds.
Stable isotope labeling allows researchers to study metabolic pathways in vivo in a safe manner.
Relying on a professional technical team and high-quality and efficient services, BOC Sciences provides stable isotope labeling with amino acids in cell culture (SILAC) services for cell culture amino acids, and can meet the needs of different isotope-labeled amino acids required for experiments.
Stable isotope labeling with amino acids in cell culture (SILAC) technology is based on the principle that cell growth depends on essential amino acids, and uses stable isotope-labeled (13C, 15N, 2H) essential amino acids to metabolically label cellular proteins.
13C, 15N, 2H are stable isotopes without radioactivity, so they can be tested under normal conditions. Arginine, lysine, and leucine are the most commonly used amino acids in SILAC. Stable isotope-labeled arginine, lysine, and leucine were added to the cell culture medium to culture the cells. These stable isotope-labeled amino acids are naturally incorporated into proteins during cell proliferation. After 5-6 generations of cell proliferation, the stable isotope-labeled amino acid is completely incorporated into the newly synthesized protein of the cell to replace the original amino acid.
SILAC technology labels proteins through the cell's normal metabolic processes. The labeled protein differs in molecular weight from unlabeled protein (incubated in normal medium), but other chemical properties are the same. This difference is distinguished by means of subsequent mass spectrometry. Relative quantitative research was carried out by comparing the peak area of the signal peaks of unlabeled and isotope-labeled samples in the first-order mass spectrometry. At the same time, protein identification can also be carried out by secondary mass spectrometry.
Before starting the experiment, we will wholeheartedly provide you with an effective SILAC experimental design plan. We can provide stable isotope-labeled amino acids.
The customer provides the sample and sample information to be identified, and the sample should avoid all kinds of pollution and repeated freezing and thawing.
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