2'-Deoxyadenosine-[15N5] hydrate

2'-Deoxyadenosine-[15N5] hydrate, a labeled nucleoside with diverse research and diagnostic applications, is instrumental in investigating various biological processes. Here are key applications highlighted with a high level of perplexity and burstiness:

Stable Isotope Labeling: In the realm of mass spectrometry-based proteomics and metabolomics, 2'-Deoxyadenosine-[15N5] hydrate plays a crucial role in stable isotope labeling studies. By integrating this heavy isotope-labeled compound into organisms' nucleic acids, researchers can meticulously monitor nucleic acid metabolism and turnover dynamics. This innovative approach facilitates a granular understanding of cellular dynamics and metabolic pathways, offering unparalleled precision in biological research.

DNA Synthesis Research: Within the domain of molecular biology, this labeled compound serves as a cornerstone in unraveling the intricate mechanisms of DNA synthesis and replication. Through the utilization of 2'-Deoxyadenosine-[15N5] hydrate in replication assays, scientists can probe the incorporation of nucleotides into DNA strands, shedding light on polymerase activity and the impact of genetic mutations on DNA replication processes. This detailed analysis provides invaluable insights into fundamental biological processes at the molecular level.

Pharmacokinetic Studies: Leveraging the power of 2'-Deoxyadenosine-[15N5] hydrate in pharmacokinetic research enables precise tracking of deoxyadenosine analog distribution and metabolism within the body. By labeling deoxyadenosine molecules, researchers can accurately measure essential pharmacokinetic parameters such as drug absorption, half-life, and clearance rates. This data plays a pivotal role in optimizing drug dosing regimens and delivery strategies, enhancing the efficacy and safety of therapeutic interventions.

Cancer Research: In the realm of oncology, 2'-Deoxyadenosine-[15N5] hydrate emerges as a critical tool for investigating the impact of specific chemotherapeutic agents on nucleic acid synthesis within cancer cells. Tracking the incorporation of this labeled nucleoside offers valuable insights into the effectiveness of nucleoside analog drugs in inhibiting tumor growth and combating cancer. This approach not only illuminates the mechanisms of action of anticancer agents but also unveils potential resistance pathways, advancing our understanding of cancer biology and treatment strategies.