As the landscape of scientific investigation constantly changes, so does the demand for new methodologies and tools that it can learn from, requiring the most forward leaning technologies possible in order to deliver them deeper insights into the biological world. Over the past few decades, stable isotope labeling services have become an invaluable tool for enabling researchers to follow molecular pathways, decipher metabolic networks, and to reveal the mechanisms of complicated ecological procedures. As a leading player in an area where new ideas are rapidly taking shape, BOC Sciences is able to offer versatile and comprehensive stable isotope labeling solutions that can meet the majority of the requirements of researchers at all different levels.

Stable Isotopes

Stable isotopes are atoms of an element that share the same number of protons, but have a varying number of neutrons in their nucleus. By contrast, stable isotopes do not decay spontaneously and are therefore perfect tracers for numerous applications. Stable IsotopeLabeling Standard stable isotopes such as carbon-13, nitrogen-15, deuterium, oxygen-18 come with their own distinctive properties that uniquely qualify them to be used in labeling experiments.

Principles of Stable Isotope Labeling

Stable isotope labeling works on the basic tenet of isotope substitution, where non-radioactive isotopes are incorporated into molecules of interest as tags. Unlike radioactive tracers, these stable isotopes (e.g. carbon 13, nitrogen 15, deuterium and oxygen 18, which have the same chemical properties as their natural counterparts) can be administered safely to humans with a negligible perturbation of normal physiological processes. This makes it possible to monitor the position, transformation, and interaction of target molecules in larger biological networks by means of labeling them selectively with isotopes. Hence, stable isotopes (when incorporated into labeled compounds such as labeled amino acids, labeled peptides, labeled nucleic acids, etc.) can serve as traceable entities. Isotope ratio changes are measured by various analytical instruments, including nuclear magnetism, mass spectrometry and gas chromatography, and the reaction pathaway/action mechanism is recognized.

Stable Isotope Labeling Services from BOC Sciences

BOC Sciences is a brand of stable isotope labeled chemicals. As a part of a worldwide supplier, BOC Sciences offers stable isotope labeled compounds to university, scientific institutions, biotechnology companies and pharmaceutical industry. Regardless of stock products or customized production services, the best solution to any needs can be provided under the professional guidance of our experienced scientists. As of today,the stable isotopic labels that we can applied are carbon 13, nitrogen 15, deuterium and oxygen 18. We are specialized in multi-step synthesis of stable isotope-labeled compounds in different labelling patterns. In addition, BOC Sciences allows isotopic labeling of any compound, and you can make labeling customization choices below.

Isotope Labels Selection

Isotope Labeled Compounds Selection

In addition, we offer various other types of custom synthesis services for isotope labeling.

Stable Isotope Labeling Services Workflow

• Contact by phone or email to receive and sign a confidentiality agreement after confirming cooperation intention.
• Responses to customer inquiry forms for radioisotope labeling services
• Estimated of services from BOC Sciences
• You usually confirm and then contact by phone or email to receive and sign a radioisotope labeling service agreement.
• Provided radioisotope labeling services. Both sides are expected to stay in close touch and collaborate during this process.
• When the project is terminated, BOC Sciences will deliver the quality inspection report, and deliver the product to the designated location by the customer.

With years of experience in the design and synthesis of stable isotopes, Isotopes provides various labeling services, including proteins, peptides, carbohydrates, nucleic acids, vitamins and other related materials and chemicals. All stages of synthesis have been mastered by our expert team and could provide a diverse range of compounds for a wide range of medicinal, and agricultural or environmentally associated purposes. The assembly, assay, identification, randomization, verification, fill/finish and GMP release are all conducted according to rigorous standards.

Advantages of Our Services

BOC Sciences prides itself on its commitment to excellence and innovation in stable isotope labeling services. With a well-trained core technical team, state-of-the-art synthesis laboratory, and advanced testing equipment, we ensure the highest quality standards at every stage of the labeling process. Our strict confidentiality system protects the confidentiality of client information, while our extensive expertise spans various synthesis stages, from production to packaging and release. With BOC Sciences as your partner, you can trust in our expertise to propel your research forward with precision and reliability.

BOC Sciences' Synthetic Expertises.

Stable isotope labeling services offered by BOC Sciences represent a transformative approach to scientific research, providing researchers with the tools and resources needed to unravel the complexities of biological systems. With our comprehensive suite of services, customizable solutions, and commitment to quality, we empower researchers to push the boundaries of knowledge and innovation in their respective fields.

Applications of Stable Isotopes

Tracing Trophic Interactions

Stable isotopes are an ecologist's best friend for following the trail of who is eating who in an ecosystem. For example, carbon-13 and nitrogen-15 isotopes show what eats what and how energy moves through food webs. Isotopic composition tells us about the ecological dynamics of organisms, from marine food chains to land environments.

Understanding Metabolic Pathways

Stable isotope applications in the study of metabolic pathways in biological systems You however can look at where the same isotopes which were used to make ATP wound up in metabolites in isotope labeling experiments which then makes glycolysis and amino acid biosynthesis much more visible. Deuterium is also very useful in the study of lipid metabolism and the rate of protein turnover, thus assisting in the characterization of many metabolic disorders and diseases.

Understanding Environmental Processes

They are very useful for looking at environmental processes and climate movements through time using stable isotopes. The isotopes oxygen-18 and hydrogen-2 are used to reconstruct paleoclimate records and understand about the climate of the past. Understanding sources and sinks of greenhouse gas emissions through the study of carbon-13 isotopes in atmospheric carbon dioxide.

Advancing Biomedical Research

Stable isotopes are used in biomedical research to investigate diseases and therapies. Stable isotope-resolved metabolomics is an isotope labeling technique that can be used to illuminate metabolic pathways associated with disease. Cancer cell-specific metabolic perturbation using 13C glucose tracing leading to discovery of target for anti-cancer therapeutic intervention.

FAQ

1: How do you know if an isotope is stable?

If an isotope is stable or not can be determined by the nucleus of the isotope as per the scientists. While, stable isotopes are isotopes, with a balanced ratio of protons to neutrons in the nucleus and can provide stable configuration. This balance prevents the nucleus from radioactive decay. Among other things, researchers determine the half-life of isotopes to evaluate the degree to which they are stable; longer half-lives generally indicate greater stability. Additionally, experimental data and theoretical models help confirm the stability of isotopes.

2: What are the stable isotopes of carbon?

The carbon stable isotopes are 12C and 13C. These are stable because the isotopes then have different amounts of neutrons, but they still do have the same amount of protons, maintaining its stability. The carbon-12 form is the most naturally abundant (98.9% of the carbon in the biosphere), followed in abundance by carbon-13 (about 1.1%).

3: What are the stable isotopes of nitrogen?

The two stable isotopes of nitrogen are nitrogen-14 (14N) and nitrogen-15 (15N). Similarly, nitrogen isotopes account for stable numbers atomic numbers of protons while differing in numbers of neutrons. Nitrogen-14 is the most predominant isotope, forming about 99.6% of naturally occurring nitrogen, whereas nitrogen-15 accounts for the remaining approximately 0.4%.

4: What is the stable isotope probing?

Stable isotope probing, or SIP, refers to the use of isotopically enriched substrates to track nutrient cycling and biodegradation in a variety of environments. This can be done by adding isotopically labeled substrates (e.g. 13C-carbon sources or 15N-nitrogen sources) to a system with microbial communities. This works not only some insights on what the functions of different microbes within the ecosystems might be.

5: How to measure stable isotopes?

These are measured using techniques from mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy to measure changes in the use of stable isotopes. Since this technique separates isotopes by mass-to-charge ratio, the ratios of different isotopes can be accurately determined using isotope dilution-mass spectrometry. Similarly, NMR spectroscopy can assess what isotope-specific chemical environments are by discerning how isotopes interact with a magnetic field In addition to these methods and spectroscopic methods like infrared spectroscopy are critical tools in many application areas as environmental science, geochemistry, and biological research.