Like many other aspects of scientific research, stable isotope labeling has evolved significantly since its initial conception, aiding in the development of breakthroughs in a wide range of disciplines. BOC Sciences is a well-organized management system of a stable isotope provider that is capable of offering custom synthesis of stable isotope-labeled compounds including deuterium, carbon 13, nitrogen 15, and oxygen 18. As a company expert in manufacture of stable isotope products, we make sure to gather a number of top experts in their own field in biology, chemistry and pharmacy and have a first-rated technical team. Our vision is to be the global leader on stable isotopes and derive our business success from providing high end isotope research solutions.

Custom Synthesis of Stable Isotope-Labeled Compounds

An isotope is an atom that has the same number of protons as another atom of the same element does, but has a different number of neutrons and thus a different atomic mass. Isotopes can be categorised as radioisotopes and stable isotopes on the basis of their physical properties. These are radioactive isotopes (e.g. 3H, 14C). In fact they decay an emit radiation energy themselves. They are "radioactive" and exist in a half-life. Non-radioactive and stable physical properties are the advantages of stable isotopes. They occur naturally in nature in ratios (abundances) and they are completely non-toxic to the human body. These could be chemically-synthesised, tagged as a drug molecule, and followed — and measured — using the necessary analytical instruments.

Stable isotope-labeled compounds have indeed turned out to be a valuable tool in studying drug metabolism, in determining cause of toxicity and in predicting possible toxicity of new compounds. It is widely used in pharmacy for isotopic labeling of stable isotopes (mostly deuterium, carbon 13, nitrogen 15, and oxygen 18) as well as for the preparation of internal standards containing stable isotope for analysis or measuring sample, by NMR or mass spectrometer for example.

Stable isotopo labelling synthesis is a laborious chemical synthesis technique. The professional team of BOC Sciences is proficient in researching stable isotope products and is dedicated to bypassing the technical barricade of isotope-labeled synthesis. Facing with the market demand, BOC Sciences team launched the development of stable isotope (2H, 13C, 15N, 18O) reagents and intermediates, and initiated the custom synthesis service of stable isotope-labeled samples- all in the hope of facilitating the research in drug screening and identification and structural determination of ingredients.

Custom Isotope Labeled Compounds

Custom Isotopic Labeling

For more exclusive customization, please feel free to contact us directly.

How We Proceed Isotope Synthesis Service?

Every custom synthesis project at BOC Sciences is subject to extensive analysis by our experts. Upon an in-depth evaluation of the client needs, we kickstart an involved R&D process to establish a synthesis route that fits the desired end-product. Utilizing their experience, our chemists suggest specific isotopic site placement in the development design stage, allowing for sensitive, stable compounds. Through the synthesis, we ensure that clients are in the loop at all stages and they hear from us on a weekly basis or sooner with regular updates on the progress of the project, and also confirm their expectations helping keep the project on track. We provide an array of ancillary services, including full analytical support and documentation, alongside the same commitment to excellence in synthesis. With the aid of highly accurate analytical techniques such as HPLC-UV-MS, mass spectrometry and NMR spectroscopy, BOC Sciences offers customized isotope-labeled compounds with the highest level of purity. With every item comes an extensive Certificate of Analysis, affirming to customers that the product is both credible and in quality condition. Our services are able to scale quantities from milligrams to grams for a wide range of research needs, and they are performed with the highest precision and accuracy.

• Custom Stable Isotope-Labelled Compound
• Manufacturing Scale from Milligrams to Grams
• Detailed Analysis and Identification Report
• Regular Updates on Your Synthesis Progress
• Strict Quality Control with GMP or non-GMP Options
• Perfect After-Sales Service

Stable lsotope-Labeled Compounds in Research

Stable isotope-labeled compounds, characterized by the incorporation of stable isotopes such as deuterium, carbon 13, nitrogen 15, and oxygen 18, find extensive utility across diverse scientific disciplines. Their unique properties and versatility make them invaluable tools in advancing research and understanding complex phenomena.

Applications of stable lsotope-labeled compounds.

Why Outsource Your Isotopes by BOC Sciences?

Relying on a first-class technical team and advanced equipment, BOC Sciences has rich experience in R&D and production of isotopic compounds. We offer a wide variety of stable isotope-labeled compounds, which are constantly being developed. We can synthesize different types of stable isotope compounds with high quality and quantity according to the specified requirements of customers. In addition, we can help design the required stable isotope compounds according to the customer's experimental project, and complete the synthesis quickly and accurately. So far, we have completed the synthesis of various isotope-labeled compounds for global pharmaceutical R&D companies and university research institutes, becoming a reliable supplier of stable isotope labels.

• Strict Quality Control System
• Professional and Efficient Team
• High-Abundance and High-Purity products
• Complete HPLC, LC-MS, GC-MS, NMR and Other Data Reports
• Timely Delivery
• Competitive Price
• Strict Customer Information Confidentiality System
• Free Technical Consultation

Case Study

Synthesis and Incorporation of 13C-Labeled DNA Building Blocks to Probe Structural Dynamics of DNA by NMR

Synthesis of stable isotope labeled DNA phosphoramidites 26, 27, 28 and 29. (Felix, N.; et al, 2017)

In this study, the synthesis of atom-specifically 13C-modified DNA building blocks enabled the precise investigation of structural and dynamic features via NMR spectroscopy. By incorporating 6-13C-modified pyrimidine and 8-13C purine DNA phosphoramidites into DNA strands, researchers were able to observe site-specific resonance assignments in a polypurine tract DNA/RNA hybrid duplex. This approach facilitated the identification of micro- to millisecond dynamics in the mini-cTAR DNA, revealing an exchange process in the lower stem of the hairpin that is enhanced by the HIV-1 nucleocapsid protein 7. Additionally, site-specific 8-13C-2' deoxyguanosine labeling allowed for the monitoring of slow exchange between two G-quadruplex folds and the re-equilibration of fold distribution post a T-jump, with a rate of 0.012 min^-1. Moreover, 13C-ZZ-exchange spectroscopy characterized the kinetics of two stacked X-conformers in a Holliday junction mimic, determining forward and backward rate constants at 25°C as 3.1 s^-1 and 10.6 s^-1, respectively. These findings underscore the power of 13C-modified nucleotides in elucidating the dynamic behavior of complex DNA structures critical in biological processes.

In this work, systematically atom-specifically 13C-labeled DNA building blocks were synthesized that allowed a detailed NMR structural and dynamic analysis. Researchers incorporated 6-13C, 8-13C DNA phosphoramidites modified at pyrimidine and purine positions in DNA strands and were able to qualitatively assign peaks to specific sites in a RNA-DNA hybrid duplex with a polypurine tract. This strategy allowed for the detection of micro- to millisecond dynamics in the mini-cTAR DNA, finding that the stem of this hairpin exchanges on a slower timescale and is further perturbed by HIV-1 nucleocapsid protein 7. Site-specific8-13C-2'-deoxyguanosine labeling further enabled slow exchange between two G-quadruplex folds and re-equilibration of fold distribution post a T-jump (k = 0.012 min^-1). In addition, 13C ZZ-exchange spectroscopy, which characterizes the kinetics of two stacked X-conformers in a Holliday junction mimic, identified forward and backward rate constants at 25°C as 3.1 s^-1 and 10.6 s^-1 respectively. ConclusionsREs derived from 13 C-labeled monomers are a novel and powerful approach to probing the dynamic behavior of the transient structures in DNA playing a key role in biological processes.

FAQ

1. What is an isotope in chemistry?

In chemistry, isotopes are different forms of an element that contain the same number of protons but a different number of neutrons within their atomic nuclei. The differences in neutron count is what can make two samples of a single element have different atomic masses. Nut theses isotopes have same chemical properties but different in mass so their physical properties will be different.

2. How to calculate atomic mass of isotopes?

The atomic mass of isotopes is calculated by a weighted average according to their natural abundance. Each isotope is multiplied by its mass and that answer is added together. Suppose, for example, that we have carbon-12 with an abundance of 98.9% and carbon-13 with an abundance of 1.1% then we add each of their mass and then multiply the sum with its respective abundance.

3. How to write isotopes?

Isotope notation is concise, typically featuring the element's symbol, followed by the mass number as a superscript, and the atomic number as a subscript. For example, carbon 12 is written as ¹²₆C, where ¹² represents the mass number and ₆ represents the atomic number.

4. How to find abundance of isotopes?

One of the relative abundance of the isotopes can be determined using mass spectrometry, which tells you how much of each isotope is present. Alternatively you can look up scientific literature or databases that keep record of isotopic abundances.