2-Hydroxyglutaric Acid-[d3] Disodium Salt

2-Hydroxyglutaric Acid-[d3] Disodium Salt is a deuterated biochemical tool used in various scientific research applications. Here are some key applications:

Metabolomics: In metabolomics research, 2-hydroxyglutaric acid-[d3] disodium salt is often used as an internal standard for quantifying 2-hydroxyglutarate levels in biological samples. Its isotopic labeling allows for accurate mass spectrometry analysis, providing insights into cellular metabolism and metabolic disorders. This application is particularly useful in studying cancer metabolism and enzyme deficiencies like 2-hydroxyglutaric aciduria.

Biochemical Pathway Tracing: Researchers utilize 2-hydroxyglutaric acid-[d3] disodium salt to trace biochemical pathways and track metabolic flux within cells. By incorporating this compound, scientists can differentiate between endogenous and exogenous 2-hydroxyglutarate, enhancing the understanding of its role in various pathways. This tool aids in uncovering new insights into cellular metabolic functions and disease mechanisms.

Chemical Synthesis Studies: In the realm of chemical synthesis, 2-hydroxyglutaric acid-[d3] disodium salt serves as a reference compound for the production and analysis of deuterated derivatives. Its stable isotopic composition allows chemists to study reaction mechanisms and optimize synthesis protocols for pharmaceutical applications. This is crucial for the development of deuterium-labeled drugs and stable isotopic tracers.

NMR Spectroscopy: 2-Hydroxyglutaric acid-[d3] disodium salt is used as a labeling reagent in NMR spectroscopy to investigate molecular structure and dynamics. Its deuterium labeling enhances the resolution and sensitivity of NMR signals, facilitating detailed analysis of biochemical interactions and conformational changes. This application is vital for studying protein-ligand interactions and complex biological systems.