2-Ketobutyric-1-[13C] acid

2-Ketobutyric-1-[13C] acid, a stable isotope-labeled compound widely utilized in scientific research, finds diverse applications across various fields. Here are key applications highlighted with a high degree of perplexity and burstiness:

Metabolic Tracing: Delving into metabolic tracing experiments, scientists harness the power of 2-Ketobutyric-1-[13C] acid to probe intricate biochemical pathways. By integrating this labeled compound into cellular systems, researchers embark on a journey to track the flow of carbon through a myriad of metabolic processes, unraveling the complexities of metabolic fluxes and comprehending the dynamic carbon utilization within cells.

NMR Spectroscopy: In the realm of nuclear magnetic resonance (NMR) spectroscopy, this compound stands as a fundamental tool for scrutinizing the enigmatic world of complex biological molecules. The distinctive [13C] labeling bestowed upon the compound offers a unique spectroscopic signature, enabling in-depth explorations of molecular structures and interactions. These studies are imperative for gaining profound insights into molecular dynamics and elucidating intricate molecular conformations.

Stable Isotope Labeling: Pivotal in the realm of proteomics, 2-Ketobutyric-1-[13C] acid emerges as a cornerstone for stable isotope labeling endeavors. Acting as a precursor for such labeling, this compound plays a crucial role in the analysis of proteins synthesized in the presence of labeled amino acids, utilizing mass spectrometry for comprehensive examination. This innovative approach is indispensable for quantitative proteomics, facilitating the detection of variations in protein expression and modifications, paving the way for a deeper understanding of cellular mechanisms.

Biochemical Research: Embarking on biochemical research adventures, scientists turn to 2-Ketobutyric-1-[13C] acid as a beacon for insights into amino acid metabolism. This compound serves as a key player in investigations of enzymatic reactions and metabolic pathways involving ketobutyrate. Through meticulous scrutiny of the incorporation and conversion of the labeled substrate, researchers navigate the intricate landscape of enzyme kinetics, deciphering the roles of specific enzymes in metabolism. This knowledge serves as a cornerstone for understanding metabolic disorders and crafting innovative therapeutic strategies to combat them effectively.