Alpha-ketoisovaleric acid-[Dimethyl-13C2] Sodium salt

Alpha-ketoisovaleric acid-[Dimethyl-13C2] Sodium salt, a stable isotope-labeled compound utilized in scientific research, plays a pivotal role in exploring biochemical pathways. Here are key applications of Alpha-ketoisovaleric acid-[Dimethyl-13C2] Sodium salt presented with high perplexity and burstiness:

Metabolic Flux Analysis: This compound serves as an indispensable tool for delineating metabolic pathways, particularly in investigations focusing on branched-chain amino acid (BCAA) metabolism. By incorporating these labeled molecules into metabolic experiments, researchers can meticulously track the flow of carbon atoms across various biochemical reactions. This aids in unraveling the complexities of cellular metabolic networks and pinpointing metabolic bottlenecks.

Nutritional Research: In the realm of nutritional research, Alpha-ketoisovaleric acid-[Dimethyl-13C2] Sodium salt is a key player in elucidating amino acid metabolism and protein synthesis in organisms. By analyzing the isotope tracer across diverse tissues and biological samples, scientists can glean insights into the nutritional requirements and metabolic destinies of BCAAs in both health and disease. This knowledge holds significant implications for optimizing dietary supplements and designing effective therapeutic interventions.

Proteomics: Within the domain of proteomics, this labeled compound serves as a crucial ally for quantitative analysis, functioning as an internal standard. When integrated into mass spectrometry workflows, it enables precise quantification of proteins and their various modifications. This contributes to a deeper understanding of cellular protein dynamics, protein modification processes, and the identification of potential biomarkers, enhancing our knowledge of the intricate world of proteomics.

Stable Isotope Labeling in Cell Culture (SILAC): Alpha-ketoisovaleric acid-[Dimethyl-13C2] Sodium salt finds its place in SILAC, a quantitative proteomics method employed in cell culture systems. By culturing cells with this labeled compound, the stable isotope gets incorporated into newly synthesized proteins, facilitating a comparative analysis of protein expression levels and post-translational modifications under varying experimental conditions. This technique opens avenues for comprehensive exploration of protein dynamics and cellular responses.