Cytidine-[15N3]

Cytidine-[15N3], a stable isotopically labeled form of cytidine, plays a crucial role in research and diagnostics. Here are key applications of Cytidine-[15N3] presented with high perplexity and burstiness:

Metabolic Tracing Studies: By incorporating Cytidine-[15N3] into metabolic pathways, researchers can meticulously track nucleotide metabolism within cells. This labeled nucleotide allows for real-time monitoring of RNA and DNA synthesis and degradation, providing deep insights into nucleotide dynamics. Such studies are pivotal for unraveling the intricate metabolic alterations seen in conditions like cancer, shedding light on the underlying mechanisms driving these changes.

RNA Synthesis Research: Scientists utilize Cytidine-[15N3] to delve into the world of RNA synthesis and turnover rates in cellular systems. When cells are supplied with Cytidine-[15N3], the isotope seamlessly integrates into the newly synthesized RNA, enabling detailed investigations into RNA polymerization and degradation processes. These studies are instrumental in deciphering the intricacies of gene expression regulation and shedding light on RNA-related disorders, offering a deeper understanding of cellular mechanisms at play.

Mass Spectrometry Analysis: Acting as an internal standard in mass spectrometry, Cytidine-[15N3] facilitates precise quantification of cytidine and its derivatives in complex biological samples. The isotopic labeling of cytidine allows for accurate discrimination and measurement in mass spectrometry analyses, enhancing the reliability of nucleoside quantification. This heightened accuracy is critical in pharmacokinetics and metabolomics studies, providing researchers with a robust tool for detailed analysis of nucleoside dynamics in biological systems.

Nucleic Acid Research: Cytidine-[15N3] finds extensive use in labeling nucleic acids for a range of experimental applications, including nucleic acid hybridization and binding studies. By incorporating this labeled cytidine, researchers can trace and characterize nucleic acid interactions, both in vivo and in vitro, offering invaluable insights into genetic expression mechanisms. These findings are pivotal for advancing our understanding of nucleic acid-based therapeutics, paving the way for innovative treatment strategies and precision medicine approaches.