Cytidine 5'-triphosphate-[5-d1,ribose-3',4',5',5''-d4] Ammonium salt

Cytidine 5'-triphosphate-[5-d1,ribose-3',4',5',5''-d4] Ammonium salt, a labeled nucleotide with diverse bioscience applications. Here are key applications presented with high perplexity and burstiness:

Nucleic Acid Research: This isotopically labeled cytidine triphosphate plays a crucial role in dissecting nucleic acid metabolism and replication processes. By integrating this labeled nucleotide into DNA or RNA, researchers can meticulously track and scrutinize the synthesis and processing of nucleic acids within cells. This application offers valuable insights into the dynamic nature of genetic information transfer and the accuracy of replication mechanisms.

Isotope Tracing Studies: The deuterium-labeled cytidine triphosphate serves as a powerful tool in metabolic labeling experiments aimed at exploring cellular nucleotide pools and their utilization. Through monitoring the labeled isotopes, scientists can uncover nuances in nucleotide turnover rates, biosynthetic pathways, and cellular responses to varying stimuli. This knowledge is pivotal for unraveling the intricacies of cellular energy dynamics and metabolic processes.

Structural Biology: In the realm of structural biology, this compound finds its niche in crystallography and NMR studies, facilitating the elucidation of conformational changes within nucleic acid structures. By incorporating labeled nucleotides, researchers gain deep insights into the intricate interactions and dynamics of nucleic acids with proteins or ligands. This application propels our understanding of molecular recognition mechanisms and regulatory processes.

Pharmaceutical Development: The labeled cytidine triphosphate analog emerges as a valuable asset in the realm of drug discovery and development. Utilizing this compound in assay systems empowers scientists to explore the impact of potential drugs on nucleic acid synthesis and polymerase activity. This application aids in pinpointing and validating novel therapeutic targets, enhancing the efficiency of drug development pathways and paving the way for groundbreaking pharmaceutical advancements.