D-altrose-[1,3-13C2]

D-altrose-[1,3-13C2], a distinguished labeled isotope of the sugar D-altrose, finds extensive use in scientific research due to its exceptional characteristics. Here are the key applications of D-altrose-[1,3-13C2]:

Metabolic Flux Analysis: D-altrose-[1,3-13C2] is strategically integrated into metabolic studies to meticulously track carbon flux across various metabolic pathways. By following the isotope’s journey, researchers gain detailed insights into cellular metabolism dynamics. This understanding is crucial for exploring how cells manage and distribute resources, advancing our knowledge of metabolic disorders and enabling bioengineering innovations.

Structural Biology: In nuclear magnetic resonance (NMR) spectroscopy, D-altrose-[1,3-13C2] becomes a pivotal tool for elucidating the three-dimensional structures of carbohydrates and their derivatives. The inclusion of 13C isotopes enhances the precision and sensitivity of NMR signals, facilitating accurate determination of molecular configurations. This application is essential for drug design and the development of carbohydrate-based therapeutic innovations.

Enzyme Mechanism Studies: D-altrose-[1,3-13C2] plays a central role in investigating the catalytic mechanisms of enzymes involved in carbohydrate metabolism. By observing interactions between the labeled sugar and enzymes, scientists uncover detailed aspects of substrate binding and the sequential steps of enzymatic reactions. This insight aids in designing enzyme inhibitors and understanding biochemical processes at a molecular level.

Glycosylation Pathway Analysis: In glycoprotein research, D-altrose-[1,3-13C2] is invaluable for dissecting glycosylation pathways and mechanisms. The labeled sugar acts as a guide in tracing the incorporation of altrose into glycoproteins during synthesis. This analysis is vital for understanding the roles of glycosylation in cell signaling, disease progression, and the development of biopharmaceuticals.