D-glucitol-[1,6-13C2]

D-glucitol-[1,6-13C2], also referred to as isotopically labeled sorbitol, plays a pivotal role in various research domains. Here are the key applications of D-glucitol-[1,6-13C2]:

Metabolic Flux Analysis: Utilizing D-glucitol-[1,6-13C2], researchers delve into metabolic flux analysis to trace the intricate movement of carbon within metabolic pathways. By integrating this labeled compound into cellular systems, scientists can meticulously monitor its dispersion and breakdown, providing profound insights into cellular metabolism. This knowledge is paramount for unraveling the complexities of metabolic disorders and fine-tuning metabolic engineering strategies.

Nuclear Magnetic Resonance (NMR) Studies: The presence of a 13C label in D-glucitol-[1,6-13C2] renders it an exceptional tool for probing through NMR spectroscopy. Researchers leverage this compound to investigate the structure and dynamics of carbohydrates along with their interactions within biological systems. It furnishes detailed insights into molecular conformation and interactions, aiding in the elucidation of intricate biological processes.

Mass Spectrometry: D-glucitol-[1,6-13C2] holds significant value in mass spectrometry for precise quantitative analysis. The isotopic labeling facilitates accurate measurement of metabolic intermediates and products in complex biological samples. This level of precision is critical for the in-depth study of metabolic pathways and regulatory mechanisms at the molecular level.

Enzyme Kinetics: Within the realm of enzymology, D-glucitol-[1,6-13C2] acts as a crucial substrate for investigating enzyme-catalyzed reactions involving sorbitol. By tracking the labeled carbon atoms, researchers gain intricate insights into enzyme mechanisms, kinetics, and specificity. This information is indispensable for comprehending enzyme function as well as designing inhibitors or modulators for therapeutic interventions.