D-glucose-[2-13C,3-d]

D-glucose-[2-13C,3-d], an isotopically labeled glucose molecule, finds diverse applications in scientific research. Here are the key applications of D-glucose-[2-13C,3-d]:

Metabolic Flux Analysis: Serving as a cornerstone in metabolic flux analysis, D-glucose-[2-13C,3-d] plays a pivotal role in elucidating glucose metabolism pathways. By integrating this labeled molecule into cells or organisms, scientists embark on a journey to trace carbon incorporation through intricate metabolic networks. This data is crucial for deciphering cellular metabolic states and pinpointing potential targets for metabolic engineering.

Magnetic Resonance Imaging (MRI) and Spectroscopy: Harnessing the power of D-glucose-[2-13C,3-d] as a tracer, MRI and NMR spectroscopy dive into the realm of physiological and biochemical processes. The labeled isotopes of this glucose variant offer a window into the detailed visualization of glucose distribution and utilization within tissues. This application shines brightly in monitoring glucose uptake in cancer research and diabetes studies, shedding light on critical metabolic pathways.

Stable Isotope Labeling in Proteomics (SILAC): Embarking on proteomic journeys, researchers unfold the potential of D-glucose-[2-13C,3-d] in stable isotope labeling experiments within cell cultures. This technique, known as SILAC, provides a quantitative lens to analyze protein dynamics by integrating labeled glucose into cellular proteins. Insights into protein synthesis, degradation rates, and post-translational modifications emerge, painting a vivid picture of protein behavior within cells.

Glycosylation Studies: Navigating the intricate landscape of glycosylation processes, D-glucose-[2-13C,3-d] emerges as a vital tool in unraveling the mysteries within cells. By tracking the incorporation of labeled glucose into glycoproteins, researchers embark on a journey to investigate glycosylation pathways and their efficiency. This exploration deepens our understanding of the role glycoproteins play in health and disease, opening doors to potential therapeutic interventions and avenues for further research.