D-fructose-[4,6-13C2]

D-fructose-[4,6-13C2], a carbon-13 labeled sugar molecule, plays a pivotal role in metabolic and biochemical research. Here are the key applications of D-fructose-[4,6-13C2]:

NMR Spectroscopy: Utilizing D-fructose-[4,6-13C2] in nuclear magnetic resonance (NMR) spectroscopy unveils the intricate metabolic pathways and fluxes within cells. The 13C label enables researchers to meticulously track the incorporation of fructose into diverse metabolic intermediates. This technique is indispensable for unraveling the dynamic nature of carbohydrate metabolism and its intricate regulation in varying biological milieus.

Metabolic Flux Analysis: In the realms of metabolic engineering and systems biology, D-fructose-[4,6-13C2] serves as a cornerstone for metabolic flux analysis (MFA). By charting the journey of labeled carbon atoms through metabolic networks, scientists can quantify the rates of metabolic reactions. This quantitative data is vital for fine-tuning metabolic pathways in bioengineered organisms, specially tailored for industrial pursuits like biofuel and bioproduct generation.

Isotope Tracing Studies: Engaging D-fructose-[4,6-13C2] in isotope tracing studies offers a glimpse into the destiny of fructose in metabolic processes. Researchers can meticulously monitor the distribution of labeled carbon within various biomolecules, unraveling insights into nutrient utilization and metabolic well-being. This methodological approach aids in comprehending metabolic disorders like diabetes and obesity by shedding light on the fate of fructose in biological systems.

Nutritional Research: In the realm of nutritional research, D-fructose-[4,6-13C2] serves as a valuable tool for investigating the repercussions of dietary fructose on human metabolism. By introducing labeled fructose to subjects, researchers can meticulously track its assimilation, conversion, and disposal within the body. These studies play a pivotal role in formulating dietary guidelines and unraveling the metabolic implications of fructose consumption, offering crucial insights into human health and nutrition.