D-fructose-[6-13C,6,6'-d2]

D-fructose-[6-13C,6,6'-d2], a compound labeled with stable isotopes, is extensively utilized in biochemical and metabolic research. Here are the key applications of D-fructose-[6-13C,6,6'-d2]:

Metabolic Flux Analysis: In the realm of metabolic studies, D-fructose-[6-13C,6,6'-d2] serves as a valuable tool for tracing the intricate pathways of carbon within cellular metabolic networks. By analyzing the distribution of labeled carbon atoms, researchers can accurately quantify fluxes in essential processes such as glycolysis and the pentose phosphate pathway. This sheds light on the complexities of cellular metabolism and pinpointing potential metabolic bottlenecks.

NMR Spectroscopy: With its incorporation of 13C and deuterium labels, D-fructose-[6-13C,6,6'-d2] emerges as a prime candidate for Nuclear Magnetic Resonance (NMR) spectroscopy investigations. Researchers can delve into the dynamics and interactions of this molecule within multifaceted biological systems, unveiling detailed insights into molecular structure dynamics and interactions at a minute atomic level.

Stable Isotope Tracing: Deployed in a myriad of stable isotope tracing experiments, D-fructose-[6-13C,6,6'-d2] offers a window into the fate of fructose molecules in diverse biological scenarios. This compound enables precise tracking of fructose metabolism in tissues and biofluids, illuminating its implications in health and disease contexts. This research holds particular relevance in the study of metabolic disorders like diabetes and obesity, providing crucial insights into disease mechanisms.

Enzyme Mechanism Studies: At the forefront of enzyme research, D-fructose-[6-13C,6,6'-d2] is instrumental in unraveling the intricate mechanisms of enzymatic reactions. By acting as a substrate in enzyme-catalyzed processes, this compound with isotope labels facilitates the exploration of kinetic isotope effects and transition states within enzymatic pathways. Understanding these nuances is pivotal for developing enzyme inhibitors and optimizing industrial biocatalysts, driving advancements in biocatalysis.