D-fructose-[6-13C] 1,6-bisphosphate sodium salt

D-fructose-[6-13C] 1,6-bisphosphate sodium salt, a labeled compound for metabolic research, sheds light on the glycolytic pathway and carbon flux. Here are the key applications:

Metabolic Flux Analysis: Employing D-fructose-[6-13C] 1,6-bisphosphate sodium salt in metabolic flux analysis allows researchers to follow the intricate carbon atom flow within glycolysis. By integrating this labeled compound into cellular systems, scientists can monitor shifts in isotopic enrichment in downstream metabolites. This methodology enables the precise quantification of metabolic pathway activity, offering insights into cellular metabolism dynamics under varying conditions.

Nuclear Magnetic Resonance (NMR) Studies: In the realm of NMR spectroscopy, this labeled compound is pivotal in exploring the structural dynamics of metabolic intermediates within the glycolytic pathway. The distinctive [6-13C] label provides a specific signal detectable and quantifiable in NMR experiments. This application serves as a cornerstone for unraveling enzyme mechanisms and deciphering the intricate regulatory networks governing metabolic processes.

Stable Isotope-Resolved Metabolomics (SIRM): At the forefront of metabolomics research, D-fructose-[6-13C] 1,6-bisphosphate sodium salt plays a key role in SIRM, a cutting-edge technique merging stable isotope labeling with mass spectrometry for metabolic pathway analysis. By tracking the incorporation of labeled carbon into diverse metabolites, researchers glean comprehensive insights into metabolic fluxes and pathway activities. Such detailed assessments are invaluable for decoding disease metabolism intricacies and formulating targeted therapeutic interventions.

Enzyme Kinetics Studies: Fascinated by enzyme kinetics, researchers leverage D-fructose-[6-13C] 1,6-bisphosphate sodium salt to delve into the intricacies of glycolysis-associated enzymes. By furnishing a labeled substrate, the kinetic properties of pivotal enzymes like aldolase and phosphofructokinase can be meticulously examined. This data is indispensable for characterizing enzyme behaviors and devising modulators like inhibitors or activators to fine-tune metabolic pathways in a precise manner.