D-Alanine-[2-13C,15N]

D-Alanine-[2-13C,15N], an isotopically labeled amino acid commonly utilized in research, offers unique insights into metabolic and biochemical processes. Below are key applications of D-Alanine-[2-13C,15N] presented with high perplexity and burstiness:

Metabolic Flux Analysis: Within the realm of metabolic research, D-Alanine-[2-13C,15N] acts as a powerful tracer, shedding light on amino acid metabolism and pathways by tracing labeled atoms through intricate metabolic networks. This approach provides a nuanced understanding of carbon and nitrogen flow in cellular processes, aiding in deciphering metabolic regulation and pinpointing bottlenecks within metabolic pathways.

Protein Turnover Studies: In the domain of proteomics, D-Alanine-[2-13C,15N] plays a crucial role in measuring protein synthesis and degradation rates within organisms. By incorporating this labeled amino acid into nascent proteins, researchers can monitor the synthesis, modification, and breakdown of proteins over time. These studies are imperative for unraveling the dynamic nature of proteins in both health and disease contexts.

Structural Biology: Encompassing structural biology, D-Alanine-[2-13C,15N] serves as a valuable label for elucidating protein structures via nuclear magnetic resonance (NMR) spectroscopy. The isotopic enrichment enhances spectral resolution, enabling detailed analysis of protein folding dynamics and interactions. This aids in unraveling the intricate relationships between protein structure and function, offering valuable insights into protein behavior and complex formation.

Microbial Ecology: In the field of microbial ecology, D-Alanine-[2-13C,15N] is a pivotal tool for exploring nitrogen assimilation and carbon turnover within microbial communities. By introducing this labeled substrate into environmental samples, researchers can track its integration into microbial biomass, providing valuable insights into microbial contributions to nutrient cycling and ecosystem dynamics. This application sheds light on the intricate roles microbes play in shaping ecosystem functions and sustainability.