D-fructose-[4-13C]

D-fructose-[4-13C], a stable isotope-labeled fructose commonly utilized in metabolic research and diagnostic applications. Below are the key applications of D-fructose-[4-13C]:

Metabolic Flux Analysis: Employing D-fructose-[4-13C] in metabolic flux analysis enables researchers to delve into carbohydrate metabolism intricacies within cells. By meticulously tracking the labeled carbon atom's journey through metabolic pathways, detailed insights into fructose utilization dynamics and conversion processes are uncovered. This analysis is instrumental in unraveling diseases associated with carbohydrate metabolism such as diabetes and metabolic syndrome.

Nutritional Studies: Within nutritional research, D-fructose-[4-13C] plays a crucial role in studying the absorption and metabolism of dietary fructose. Through controlled experiments involving labeled fructose administration, researchers can precisely measure its metabolic efficiency and its impact on blood sugar levels. This information is pivotal for shaping dietary recommendations and comprehending the nuanced relationship between fructose and health outcomes.

Medical Diagnostics: The utilization of D-fructose-[4-13C] in breath tests for diagnosing conditions like fructose malabsorption showcases its versatility in medical diagnostics. Patients ingest the labeled fructose, and the detection of metabolic byproducts in their breath offers a non-invasive method of assessing fructose digestion. This aids in the identification of digestive disorders and tailoring personalized dietary interventions for affected individuals.

Enzyme Kinetics Studies: In the realm of biochemical research, D-fructose-[4-13C] serves as a potent tool for delving into the kinetics of enzymes associated with fructose metabolism. By employing isotopically labeled substrates, scientists can interrogate enzyme mechanisms and ascertain reaction rates under diverse conditions. This knowledge proves invaluable for drug development endeavors and deepening our understanding of metabolic disorders at a molecular level.