D-Glucose-[5-13C]

D-Glucose-[5-13C], a stable isotope-labeled glucose molecule, is a versatile tool with wide-ranging applications in scientific research. Here are the key applications of D-Glucose-[5-13C]:

Metabolic Flux Analysis: In the intricate realm of cellular metabolism, researchers heavily rely on D-Glucose-[5-13C] for metabolic flux analysis. By tracing its intricate journey through metabolic products, scientists can intricately map the flow of carbon atoms across diverse metabolic networks. This method reveals profound insights into cell metabolism, metabolic disorders, and the subtle adjustments required for fine-tuning metabolic engineering strategies.

Magnetic Resonance Imaging (MRI): Stepping into the domain of MRI studies, the utilization of D-Glucose-[5-13C] enriches the visualization of in vivo metabolic processes. The 13C isotope empowers high-resolution imaging of glucose metabolism within tissues and organs, illuminating disease conditions like cancer characterized by disrupted glucose metabolism. This application emerges as a pivotal cornerstone in unraveling the complexities of metabolism.

Stable Isotope Resolved Metabolomics (SIRM): Within the expansive realm of SIRM studies, D-Glucose-[5-13C] assumes a central role in unveiling metabolic pathways and quantifying fluxes within cells and tissues. This unraveling of intricate biological processes helps shed light on metabolic alterations under diverse physiological and pathological contexts, offering invaluable insights into disease mechanisms and potential avenues for therapeutic interventions.

Biochemical Assays: Playing a critical role in biochemical assays, D-Glucose-[5-13C] emerges as a key protagonist in measuring enzyme activities and metabolic rates in vitro. By incorporating this labeled glucose into enzymatic reactions, researchers can meticulously quantify the production of a myriad of metabolites essential for deciphering enzyme kinetics and the orchestration of metabolic pathways. This information serves as a foundational pillar in deciphering the nuances of metabolic regulation.