D-Glucose-[6,6-d2]

D-Glucose-[6,6-d2], a deuterated variant of glucose commonly utilized in metabolic research and diverse scientific investigations, offers a plethora of applications. Here are the key applications:

Metabolic Tracing: Delving into the intricate web of glucose metabolism pathways, D-Glucose-[6,6-d2] plays a pivotal role in metabolic tracing experiments. By infusing this labeled glucose into biological systems, researchers embark on a journey to trace its metabolic destiny and quantify tissue-specific glucose utilization. This methodology is indispensable for unraveling the mysteries of disorders such as diabetes and metabolic syndrome.

Cell Culture Studies: Within the realm of cell culture investigations, D-Glucose-[6,6-d2] emerges as a metabolic substrate of choice for probing cellular glucose uptake and utilization dynamics. Researchers venture into the realm of understanding how various cell types metabolize glucose and respond to metabolic perturbations or stimulations. This exploration sheds light on the metabolic adaptations exhibited by cancer cells, stem cells, and other specialized cell populations.

NMR Spectroscopy: Harnessing the power of nuclear magnetic resonance (NMR) spectroscopy, D-Glucose-[6,6-d2] steps into the spotlight for studying carbohydrate metabolism and enzyme kinetics. The presence of deuterium atoms imparts unique spectral signatures that aid in characterizing glucose derivatives and their metabolic transformations. This technique serves as a cornerstone for in-depth metabolic profiling and structural analysis of intermediary metabolites.

In Vivo Studies: Embracing the domain of animal models, in vivo studies with D-Glucose-[6,6-d2] illuminate the systemic intricacies of glucose metabolism encompassing production and disposal rates. By administering this labeled glucose in vivo, researchers embark on a comprehensive exploration of whole-body glucose handling mechanisms. This avenue of exploration proves invaluable for deciphering the nuances of metabolic disorders and forging new paths in therapeutic interventions.