DL-Histidine-[ring-2-13C]

DL-Histidine-[ring-2-13C] is an isotopically labeled amino acid used in various advanced research applications. Here are some key applications of DL-Histidine-[ring-2-13C]:

Metabolic Flux Analysis: DL-Histidine-[ring-2-13C] is utilized in metabolic flux analysis to trace the metabolic pathways and fluxes of histidine in biological systems. By tracking the 13C label through different metabolites, researchers can gain insights into histidine metabolism and its regulation. This information is crucial for understanding metabolic disorders and optimizing metabolic engineering efforts.

Protein Structure and Dynamics: In the field of structural biology, DL-Histidine-[ring-2-13C] is used in NMR (Nuclear Magnetic Resonance) spectroscopy to study protein structures and dynamics. The incorporation of the 13C label into histidine residues allows scientists to precisely monitor histidine environments in proteins. This is essential for elucidating protein folding, conformational changes, and interactions with other molecules.

Enzyme Mechanism Studies: DL-Histidine-[ring-2-13C] is employed to investigate enzyme mechanisms, particularly those involving histidine residues in catalytic sites. By analyzing the 13C-labeled histidine in enzyme-substrate complexes, researchers can discern detailed mechanistic steps and intermediate states. This understanding aids in the design of enzyme inhibitors or activators for therapeutic purposes.

Isotopic Labeling in Biomolecule Synthesis: DL-Histidine-[ring-2-13C] is integral in synthesizing isotopically labeled peptides and proteins for various research applications. The labeled histidine can be incorporated into target proteins, enabling detailed studies in mass spectrometry and other analytical techniques. This approach supports research in drug discovery, proteomics, and understanding complex biological processes.