Glycine-N-Fmoc-[15N]

Glycine-N-Fmoc-[15N] is a labeled amino acid derivative that serves as a crucial tool in various biochemical and biophysical studies. Here are some key applications of Glycine-N-Fmoc-[15N]:

Nuclear Magnetic Resonance (NMR) Spectroscopy: Glycine-N-Fmoc-[15N] is used in NMR spectroscopy to study protein and peptide structure and dynamics. The [15N] label provides a unique NMR signal that enhances the resolution and sensitivity of protein studies. This application is instrumental for gaining detailed insights into molecular interactions and conformational changes.

Mass Spectrometry-based Proteomics: In proteomics, Glycine-N-Fmoc-[15N] can be incorporated into proteins to serve as an isotopic label for mass spectrometry analysis. This allows for the precise quantification and identification of peptides and proteins in complex mixtures. By facilitating differential analysis, researchers can study protein expression levels and post-translational modifications under various conditions.

Peptide Synthesis: Glycine-N-Fmoc-[15N] is employed in the synthesis of isotopically labeled peptides for biological and pharmacological research. These labeled peptides are crucial for studying peptide-receptor interactions and for developing peptide-based therapeutics. The incorporation of [15N] enables tracking and analysis of peptides in vitro and in vivo.

Stable Isotope Labeling: The compound is used in metabolic studies to trace nitrogen assimilation and metabolic pathways. By incorporating [15N] into biological systems, researchers can analyze nutrient flow and nitrogen fixation processes. This application is vital for understanding fundamental biological processes and improving agricultural practices through isotope tracing techniques.