Name: HEPES-[d18]
Brand: BOC Sciences
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General Information

Catalog: BLP-001663

CAS: 203805-87-2

Molecular Formula: C8D18N2O4S

Molecular Weight: 256.42

Chemical Structure

Description	HEPES-[d18] is the labelled analogue of HEPES. HEPES is an N-substituted aminosulfonic acid buffer.
Synonyms	4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid-d18; HEPES-d18; ACM203805872; 1-Piperazine-2,2,3,3,5,5,6,6-d8-ethane-a,a,b,b-d4-sulfonicacid-d,4-[2-(hydroxy-d)ethyl-1,1,2,2-d4]-(9ci); N-2-HYDROXYETHYLPIPERAZINE-N'-ETHANESULFONIC ACID-D18; N-(2-HYDROXYETHYL)PIPERAZINE-N'-2-ETHANESULFONIC ACID (D18); HEPES (D18); N-2-Hydroxyestrone-N-ethanesulfic Acid-D18
IUPAC Name	deuterio 1,1,2,2-tetradeuterio-2-[2,2,3,3,5,5,6,6-octadeuterio-4-(1,1,2,2-tetradeuterio-2-deuteriooxyethyl)piperazin-1-yl]ethanesulfonate
Related CAS	7365-45-9 (unlabelled)
Canonical SMILES	C1CN(CCN1CCO)CCS(=O)(=O)O
InChI	InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)/i1D2,2D2,3D2,4D2,5D2,6D2,7D2,8D2,11D/hD
InChI Key	JKMHFZQWWAIEOD-MMODJCGESA-N
Melting Point	227-233°C (dec.)
Purity	98% (CP); 98% atom D

HEPES-[d18] is a heavy isotope-labeled buffer commonly used in life sciences research for its superior buffering capacity and stability. Here are some key applications of HEPES-[d18]:

NMR and Mass Spectrometry: HEPES-[d18] is extensively used in nuclear magnetic resonance (NMR) and mass spectrometry studies as a deuterated buffer. The deuterium labeling minimizes background interference, allowing for clearer spectral data interpretation. This is particularly beneficial in elucidating molecular interactions and structural dynamics of biomolecules.

Cell Culture: In cell culture, HEPES-[d18] serves as a stable buffering agent that maintains physiological pH levels, especially in environments where CO2 regulation is not feasible. Its deuterated form ensures minimal isotopic interference in experiments using labeled compounds. This stability helps in maintaining cell viability and consistency in reproducibility of experimental results.

Biophysical Assays: HEPES-[d18] is employed in biophysical assays where accurate pH maintenance is crucial, such as enzyme kinetics and protein-ligand binding studies. The heavy isotope labeling of HEPES minimizes signal overlap in spectroscopic analyses. This makes it ideal for high-precision studies requiring isotopic clarity.

Fluorescence Microscopy: HEPES-[d18] is used in fluorescence microscopy applications due to its low autofluorescence and reliable pH buffering. The stability of HEPES-[d18] in maintaining the microenvironment’s pH is crucial for consistent fluorescence signal detection. This enhances the accuracy of live-cell imaging experiments aimed at monitoring physiological changes.