L-allo-Isoleucine-[13C6,15N]

L-allo-Isoleucine-[13C6,15N] is a stable isotope-labeled amino acid used in bioscience research. Here are some key applications of L-allo-Isoleucine-[13C6,15N]:

Metabolomics Studies: L-allo-Isoleucine-[13C6,15N] is utilized in metabolomics to study the metabolic pathways and fluxes in cells. By incorporating the labeled amino acid into cellular systems, researchers can trace its metabolic incorporation and transformation. This helps in elucidating metabolic dynamics and understanding alterations linked to various physiological and pathological conditions.

Protein Turnover Studies: The labeled isotope of L-allo-Isoleucine is instrumental in studying protein synthesis and degradation rates. By tracking the incorporation of the labeled amino acid into newly synthesized proteins, scientists can measure protein turnover rates in cells and tissues. This provides insights into protein metabolism under different conditions, such as stress, disease, and aging.

Peptide and Protein Quantification: In mass spectrometry-based proteomics, L-allo-Isoleucine-[13C6,15N] serves as an internal standard for quantifying peptides and proteins in complex biological samples. The labeled amino acid helps achieve accurate and reproducible quantification by compensating for variability in sample preparation and analysis. This is crucial for comparative studies and biomarker discovery.

Tracer Studies in Clinical Research: L-allo-Isoleucine-[13C6,15N] is used as a tracer in clinical research to investigate amino acid metabolism and nutritional needs in humans. By monitoring the labeled amino acid in blood and urine samples, researchers can assess metabolic rates and nutritional status. This information is valuable for developing dietary recommendations and understanding metabolic disorders.