L-allo-Isoleucine-[13C6]

L-allo-Isoleucine-[13C6] is a labeled amino acid used in various research and analytical applications. Here are some key applications of L-allo-Isoleucine-[13C6]:

Metabolic Flux Analysis: L-allo-Isoleucine-[13C6] is commonly used in metabolic flux analysis to study amino acid metabolism in microorganisms and mammalian cells. By tracing the incorporation of the labeled carbon isotope into metabolic intermediates, researchers can elucidate metabolic pathways and flux distributions. This information is crucial for optimizing metabolic engineering strategies and understanding cellular metabolism.

Protein Synthesis Studies: In protein synthesis research, L-allo-Isoleucine-[13C6] can be incorporated into proteins, allowing for the study of protein dynamics and turnover. By using mass spectrometry, scientists can track the labeled amino acid within newly synthesized proteins. This aids in investigating protein assembly, degradation rates, and overall protein homeostasis.

Nutritional Research: Labeled amino acids like L-allo-Isoleucine-[13C6] are utilized in nutritional studies to assess dietary protein quality and amino acid bioavailability. By administering labeled amino acids to subjects and measuring their appearance in biological samples, researchers can evaluate the efficiency of amino acid absorption and utilization. This helps in formulating dietary recommendations and understanding amino acid metabolism in different populations.

Pharmacokinetics: L-allo-Isoleucine-[13C6] can be employed in pharmacokinetic studies to investigate the absorption, distribution, metabolism, and excretion of amino acid-based drugs and supplements. By using labeled isotopes, researchers can track the movement of these compounds within the body over time. This provides valuable information for optimizing dosing regimens and improving therapeutic efficacy.