L-allo-Isoleucine-[13C6,d10,15N]

L-allo-Isoleucine-[13C6,d10,15N], a stable isotope-labeled amino acid, finds widespread application in diverse research fields. Here are the key applications

Metabolomics Research: Delving into metabolic pathways and fluxes, L-allo-Isoleucine-[13C6,d10,15N] serves as a cornerstone in metabolomics. By integrating this labeled amino acid into metabolic networks, researchers can meticulously trace biochemical transformations, unveiling the intricacies of metabolic reactions. This approach not only illuminates disease mechanisms but also unveils potential therapeutic targets, providing a comprehensive understanding of metabolic intricacies

Stable Isotope Labeling: At the core of stable isotope labeling experiments, this compound acts as a crucial tracer to monitor the fate of isoleucine in various biochemical and physiological processes. Through the incorporation of L-allo-Isoleucine-[13C6,d10,15N] into proteins, researchers gain valuable insights into protein synthesis, turnover, and degradation dynamics. This knowledge is essential for grasping protein functionality and dynamics, offering a detailed view of cellular processes

Nuclear Magnetic Resonance (NMR) Studies: Leveraging L-allo-Isoleucine-[13C6,d10,15N] in NMR spectroscopy unravels the structural dynamics and interactions of biomolecules with unparalleled clarity. The strategic isotope labeling significantly boosts the resolution and sensitivity of NMR signals, facilitating the analysis of complex molecular structures. This application, particularly valuable in structural biology and drug discovery, enables researchers to gain deeper insights into biomolecular mechanisms with a high degree of precision

Proteomics: In the realm of proteomics, L-allo-Isoleucine-[13C6,d10,15N] plays a pivotal role in quantitative mass spectrometry-based investigations. Serving as an internal standard, this amino acid aids in precise protein quantification and identification within intricate biological samples. By utilizing this compound, researchers can unravel differential protein expression patterns and decipher cellular responses under diverse conditions, paving the way for a comprehensive understanding of proteomic landscapes