L-Asparagine-[13C4,d3,15N2] monohydrate

L-Asparagine-[13C4,d3,15N2] monohydrate is a labeled amino acid used extensively in research and industrial applications. Here are some key applications of L-Asparagine-[13C4,d3,15N2] monohydrate:

Metabolic Flux Analysis: This labeled compound is crucial in conducting metabolic flux analysis (MFA) experiments. By tracing the pathways of 13C-labeled asparagine in cells, researchers can gain insights into amino acid metabolism and nutrient utilization. This information is pivotal for optimizing bacterial and yeast strains for industrial fermentation processes.

Protein Synthesis Studies: L-Asparagine-[13C4,d3,15N2] monohydrate can be used to study protein synthesis and turnover rates in both in vitro and in vivo systems. Incorporation of labeled asparagine into proteins allows for the precise identification and quantification of newly synthesized proteins. This application is particularly useful in proteomics and understanding the dynamics of protein expression.

Nutritional Research: In nutritional science, isotopically labeled amino acids like L-Asparagine-[13C4,d3,15N2] provide invaluable data on amino acid absorption, utilization, and metabolism. Studies using this compound help to track the assimilation and incorporation of dietary proteins in the body. This aids in formulating dietary supplements and optimizing nutritional strategies.

Drug Metabolism and Pharmacokinetics: The labeled compound serves as a tracer in drug metabolism and pharmacokinetic (DMPK) studies. Using L-Asparagine-[13C4,d3,15N2], scientists can investigate how drugs interact with metabolic pathways and measure the rates of drug absorption, distribution, metabolism, and excretion. This data is essential for the design and development of safer and more effective drugs.