L-Asparagine-[15N2,d8] monodeuterate

L-Asparagine-[15N2,d8] monodeuterate, a stable isotope-labeled amino acid, boasts a wide array of applications in scientific research. Here are key applications of this compound presented with high perplexity and burstiness:

Metabolic Tracing: In the intricate realm of amino acid metabolism and biosynthetic pathways, researchers wield L-Asparagine-[15N2,d8] monodeuterate for conducting metabolic tracing studies. By seamlessly integrating this labeled amino acid into metabolic processes, scientists embark on a journey of meticulous monitoring, observing its distribution and metamorphosis within cells and tissues. Through this method, critical insights into metabolic fluxes are gleaned, aiding in the precise pinpointing of deviations associated with diseases or metabolic irregularities.

Proteomics Research: Positioned at the forefront of proteomics, L-Asparagine-[15N2,d8] monodeuterate emerges as a linchpin for facilitating quantitative mass spectrometry-based analyses. Functioning as a robust internal standard, this compound enables the precise quantification of proteins and peptides within complex biological samples. By furnishing researchers with accurate measurements of protein abundance, it serves as a beacon guiding the exploration of variations in protein expression tied to both physiological and pathological states.

NMR Spectroscopy: Embracing the power of isotopic labeling bestowed by L-Asparagine-[15N2,d8] monodeuterate, scientists delve into nuclear magnetic resonance (NMR) investigations with fervor. This strategic approach unlocks the potential for meticulous exploration of protein structures, dynamics, and interactions at an atomic scale. Such applications play a pivotal role in unraveling the intricate tapestry of protein functions, folding patterns, and the design of therapeutics, honing in on specific protein interactions.

Structural Biology: Within the domain of X-ray crystallography and structural biology techniques, L-Asparagine-[15N2,d8] monodeuterate emerges as a prized possession. By endowing heavy isotope enrichment, this compound aids in resolving the intricacies found within electron density maps, heightening the precision of determining 3D protein structures. This advancement serves as a cornerstone in deepening our understanding of molecular mechanisms while streamlining the rational design of pharmaceutical interventions.