L-Asparagine-[2,3,3-d3,15N2] monohydrate

L-Asparagine-[2,3,3-d3,15N2] monohydrate is an isotopically labeled compound that finds its applications in various fields of bioscience and biochemistry. Here are some key applications of L-Asparagine-[2,3,3-d3,15N2] monohydrate:

Metabolic Flux Analysis: This isotopically labeled form of L-asparagine is used in metabolic flux analysis to trace the pathways of nitrogen and carbon in cellular metabolism. By incorporating this compound into cell cultures, researchers can follow its incorporation into metabolic intermediates and end products. This provides precise insights into metabolic networks and enzymatic activities within cells.

Protein Structure and Function Studies: L-Asparagine-[2,3,3-d3,15N2] monohydrate is used in the field of NMR spectroscopy to study protein structure and function. The incorporation of deuterium and nitrogen-15 isotopes helps in obtaining detailed NMR spectra and understanding the dynamic processes within proteins. This is pivotal for elucidating protein folding, binding interactions, and conformational changes.

Amino Acid Transport Studies: Researchers use this labeled compound to investigate amino acid transport mechanisms across cell membranes. By tracking the uptake and distribution of labeled L-asparagine, scientists can identify and characterize transporters and understand their regulation under different physiological conditions. This aids in comprehending how cells acquire and utilize essential amino acids.

Nutritional Studies: In nutritional biochemistry, L-Asparagine-[2,3,3-d3,15N2] monohydrate is employed to study amino acid assimilation and metabolism in organisms. By feeding this compound to animal models or cell cultures, researchers can assess the dietary contributions of asparagine to overall protein synthesis and metabolic health. This understanding is crucial for designing effective nutritional interventions and supplements.